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目的 探讨自体雪旺氏细胞植入胎儿神经后修复周围神经缺损的效果,寻找替代自体神经移植的材料。方法 用Wistar大鼠80只切断左侧大腿坐骨神经,造成15mm缺损,分别用自体神经(A组),液氮冷冻胎儿神经(B组),液氮冷冻胎儿神经加自体雪旺氏细胞粗制品(C组)进行桥接。于术后4、12、24周取桥体、桥体远端坐骨神经,分别行电镜、光镜观察、图像分析和电生理检测,所得数据经单因素方差分析和q检验。结果 A组和C组间有髓纤维数目、无髓纤维数目、复合动作电位峰值恢复率、传导速度恢复率均无显著差异,两组神经再生效果相近,而B组的再生效果则不及A、C两组;B组与A组,B组与C组分别作两两比较,部分指标存在显著差异(P<0.01)。结论 作者认为植入自体雪旺氏细胞的胎儿神经桥接周围神经缺损优于单纯胎儿神经桥接,是一种良好的替代自体神经的桥接物,有进一步研究价值和临床应用前景。
Objective To investigate the effect of repairing peripheral nerve defects after implantation of fetal Schwann cells into the fetal nerves and to search for alternative materials for autologous nerve graft. Methods 80 Wistar rats were used to cut the left thigh sciatic nerve, which caused a 15mm defect. The rats were sacrificed with autologous nerves (group A), liquid nitrogen frozen fetal nerves (group B), liquid nitrogen frozen fetal nerves plus autologous Schwann cells C group) for bridging. At 4, 12 and 24 weeks after operation, the distal sciatic nerve of bridge and bridge were taken and examined by electron microscope, light microscope, image analysis and electrophysiological examination respectively. The data were analyzed by one-way ANOVA and q-test. Results There was no significant difference in the number of myelinated fibers, the number of non-myelinated fibers, the peak recovery rate of composite action potential and the recovery rate of conduction velocity between groups A and C, but the regeneration effect of group B was not as good as that of group A, C and C groups; B group and A group, B group and C group were compared with each other, some of the indicators there was significant difference (P <0.01). Conclusion The authors believe that the fetal nerve implanted in Schwann cells implanted peripheral nerve defects better than simple fetal nerve bridge, is a good alternative autonomic nerve bridge, with further research value and clinical application prospects.