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目的观察钙离子与牛磺酸跨心肌细胞膜内流的相互影响。方法取新生ＳＤ大鼠心室肌制备培养搏动心肌细胞，采用放射性同位素４５Ｃａ２＋及３Ｈ－牛磺酸示踪技术。结果①牛磺酸内流在低胞外Ｃａ２＋浓度（［Ｃａ２＋］）００．５ｍｍｏｌＬ）和高［Ｃａ２＋］０（４．０ｍｍｏｌＬ）均比正常［Ｃａ２＋］０（１．５ｍｍｏｌＬ）和高［Ｃａ２＋］０（４．０ｍｍｏｌＬ）时增加，尤以低［Ｃａ２＋］０时明显；异搏定（１０μｍｏｌＬ）能促进牛磺酸的内流；②１０～２０ｍｍｏｌＬ牛磺酸对不同［Ｃａ２＋］０（０．５、１．５和４．０ｍｍｏｌＬ）下的Ｃａ２＋内流均有抑制作用，而１ｍｍｏｌＬ牛磺酸只抑制高［Ｃａ２＋］０时Ｃａ２＋内流，对低［Ｃａ２＋］０和正常［Ｃａ２＋］０时Ｃａ２＋内流无明显作用；１～２０ｍｍｏｌＬβ－丙氨酸对Ｃａ２＋内流无明显影响。结论胞外Ｃａ２＋浓度的变化可直接影响牛磺酸内流，而牛磺酸能抑制Ｃａ２＋的内流，两者相互影响，从而发挥牛磺酸调节细胞内Ｃａ２＋浓度的作用。
Objective To observe the interaction between calcium ions and taurine intramyocardial membrane influx. Methods Pulmonary ventricular myocytes from neonatal SD rats were used to prepare pulsed cardiomyocytes. The radioactive isotope 45Ca2 + and 3H-taurine were used to detect the myocardial cells. Results ① The influx of taurine was lower than that of normal [Ca2 +] 0 (1.5mmolL) in low extracellular Ca2 + concentration ([Ca2 +]) ) And high [Ca2 +] 0 (4.0 mmol L), especially when low [Ca2 +];; Verapamil (10 μmol L) promoted the influx of taurine; Sulfonic acid inhibited Ca2 + influx under different [Ca2 +] 0 (0.5, 1.5 and 4.0 mmol L), whereas 1 mmol L taurine inhibited Ca2 + only at high [Ca2 +] 0 Flow, no significant effect on Ca2 + influx at low [Ca2 +] 0 and normal [Ca2 +] 0; 1 ~ 20 mmolLβ-alanine had no significant effect on Ca2 + influx. CONCLUSION: The change of extracellular Ca2 + concentration can directly affect the influx of taurine, while taurine can inhibit the inward influx of Ca2 +, which interacts with each other, thus taurine can regulate the intracellular Ca2 + concentration.