目的建立能快速同时分离检测桑叶中芦丁、绿原酸和槲皮素的毛细管电泳法。方法采用未涂层毛细管柱(50μm×56 cm,有效长度47.5 cm),以含体积分数15%甲醇的12 mmol·L-1硼砂(pH值9.0)为运行缓冲溶液,工作电压25 kV,检测波长350 nm,柱温25℃。结果芦丁、绿原酸和槲皮素质量浓度分别在3.92~39.2 mg·L-1、5.52~55.2 mg·L-1和2.12~21.2 mg·L-1内线性关系良好,回收率和重复性良好。结论该法快速准确,适合于桑叶药材的质量控制。 OBJECTIVE To establish a rapid and simultaneous capillary electrophoresis method for the simultaneous determination of rutin, chlorogenic acid and quercetin in mulberry leaves. Methods Uncoated capillary column (50 μm × 56 cm, effective length 47.5 cm) was used as the running buffer solution with 12 mmol·L-1 borax (pH 9.0) containing 15% methanol. The working voltage was 25 kV. Wavelength 350 nm, column temperature 25 ℃. Results The linearities of rutin, chlorogenic acid and quercetin in the range of 3.92-39.2 mg · L-1, 55.52-55.2 mg · L-1 and 2.12-21.2 mg · L-1 were good, and the recoveries and repeats Good sex. Conclusion The method is fast and accurate and is suitable for the quality control of mulberry leaf medicinal materials.