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目的:观察CD137-CD137配体(CD137L)轴对载脂蛋白E基因敲除(ApoE-/-)小鼠活化T细胞核因子胞浆1型(NFATc1)表达的影响。方法:ApoE-/-小鼠动脉粥样斑块模型采用颈动脉硅胶圈植入法;分别采用免疫组化及流式细胞术检测小鼠颈动脉斑块及淋巴细胞NFATc1表达;分别应用RT-PCR和流式细胞技术检测体外培养的小鼠淋巴细胞NFATc1 mRNA和蛋白表达。结果:在体情况下应用anti-CD137特异性刺激CD137-CD137L轴后,ApoE-/-小鼠斑块及脾脏中淋巴细胞NFATc1表达增加;体外培养的淋巴细胞刺激CD137-CD137L轴后,淋巴细胞NFATc1 mRNA和蛋白表达明显上调,anti-CD137刺激浓度以20 mg/L时作用最强,作用24 h最明显(P<0.05)。应用Anti-CD137L特异性阻断CD137-CD137L轴能明显抑制NFATc1 mRNA及蛋白表达,浓度在20 mg/L时抑制最强,时间为24 h后抑制最佳(P<0.05)。结论:ApoE-/-小鼠体内NFATc1的表达受CD137-CD137L轴调控。
Objective: To observe the effect of CD137-CD137 ligand (CD137L) axis on the expression of NFATc1 in activated T cells of apolipoprotein E gene knockout (ApoE - / -) mice. Methods: The atherosclerotic plaque model of ApoE - / - mice was implanted with carotid artery silicone rings. The expression of NFATc1 in plaque and lymphocytes was detected by immunohistochemistry and flow cytometry respectively. PCR and flow cytometry were used to detect the expression of NFATc1 mRNA and protein in mouse lymphocytes cultured in vitro. RESULTS: The expression of NFATc1 in lymphocytes of ApoE - / - mice and ApoE - / - mice was increased after CD137-CD137L axis was stimulated with anti-CD137 specifically in vivo. After CD137-CD137L axis was stimulated with lymphocytes in vitro, the number of lymphocytes NFATc1 mRNA and protein expression was significantly up-regulated, anti-CD137 stimulation at a concentration of 20 mg / L strongest effect, the role of 24 h was the most significant (P <0.05). Anti-CD137L blocking the CD137-CD137L axis can significantly inhibit the expression of NFATc1 mRNA and protein. The inhibitory effect was best at the concentration of 20 mg / L and the inhibition was best at 24 h (P <0.05). Conclusion: The expression of NFATc1 in ApoE - / - mice is regulated by CD137-CD137L axis.