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为探讨水飞蓟宾对人CIK细胞杀伤胃癌细胞株BCG-823的影响及作用机制,分离健康志愿者外周血单个核细胞,体外诱导培养成人CIK细胞;收集培养扩增7d的CIK细胞,将其用不同浓度的水飞蓟宾诱导24h、48h、72h,CCK-8法检测其增殖情况;流式细胞术检测CIK细胞穿孔素、颗粒酶B、CD107a和IFN-γ的表达变化及水飞蓟宾对其凋亡的影响;LDH法检测CIK细胞对胃癌细胞株BCG-823的杀伤活性。结果显示,与对照组相比,浓度为(1.6~50)μg/mL的水飞蓟宾作用CIK细胞72h后,增殖率较对照组显著增加(P<0.05);穿孔素、颗粒酶B、CD107a和IFN-γ的表达以及活细胞率均不同程度增加(P<0.05);且对胃癌细胞BGC-823的杀伤活性显著增强(P<0.05)。以上实验结果提示一定浓度的水飞蓟宾对CIK细胞有促增殖作用,且能增强其对胃癌细胞株BCG-823的杀伤活性。
To investigate the effect of silibinin on human gastric cancer cell line BCG-823 induced by human CIK cells and the mechanism of action, we isolated the peripheral blood mononuclear cells of healthy volunteers and induced the growth of adult CIK cells in vitro. CIK cells cultured for 7 days were collected, The expression of perforin, granzyme B, CD107a and IFN-γ in CIK cells was detected by flow cytometry and the effects of water-fly The effect of thiabendazole on apoptosis of the gastric cancer cell line BCG-823 was tested by LDH assay. The results showed that compared with the control group, the proliferation rate of CIK cells treated with silybin at a concentration of (1.6-50) μg / mL for 72 hours was significantly higher than that of the control group (P <0.05); perforin, granzyme B, The expression of CD107a and IFN-γ and the percentage of viable cells increased to different extents (P <0.05). The cytotoxicity against BGC-823 in gastric cancer cells was significantly increased (P <0.05). The above results suggest that a certain concentration of silybin on CIK cells promote proliferation, and can enhance its killing activity of gastric cancer cell line BCG-823.