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本试验发现10μg／ml阿霉素可诱导人粘液表皮样癌MEC-1细胞凋亡，在用流式细胞仪（FCM）测细胞DNA变化及p53蛋白表达时，p53蛋白表达和凋亡细胞随着药物作用时间的延长而剧增，p53的免疫组化也说明了阿霉素可使细胞中p53蛋白表达增强。这些结果揭示了阿霉素诱导的MEC-1细胞凋亡为p53依赖性的。p53蛋白与细胞凋亡之间关系密切，也为基因疗法和凋亡的机理提供了一定的理论依据。
This experiment found that 10μg/ml doxorubicin can induce apoptosis of human mucoepidermoid carcinoma MEC-1 cells. When flow cytometry (FCM) was used to measure cell DNA changes and p53 protein expression, p53 protein expression and apoptotic cells were associated with With the prolonged prolongation of drug action, immunohistochemistry of p53 also demonstrated that doxorubicin can increase the expression of p53 protein in cells. These results revealed that doxorubicin-induced apoptosis of MEC-1 cells was p53-dependent. The close relationship between p53 protein and apoptosis also provides a theoretical basis for gene therapy and apoptosis mechanism.