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目的:观察替米沙坦对代谢综合征患者内皮祖细胞(EPCs)功能的影响。方法:将代谢综合征患者76例随机分为常规组和试验组各38例,同时选择健康体检者38例为对照组。试验组在常规用药的基础上加服替米沙坦80 mg/d,在治疗前及治疗后4周取外周血,密度梯度离心法分离培养EPCs,免疫荧光双染法鉴定EPCs,流式细胞仪、30 min贴壁法观察EPCs的体外增殖、粘附能力,Griess法检测一氧化氮(NO)分泌量,RTPCR半定量测定内皮型一氧化合酶(eNOS)基因表达。结果:与常规组相比,治疗后试验组EPCs的体外增殖、粘附能力明显增强,NO分泌量明显增多,eNOS基因表达明显上调,P均<0.05;而常规组相比则无明显改变。结论:替米沙坦能改善代谢综合征患者EPCs功能,这与上调eNOS基因表达,促进NO分泌有关。
Objective: To observe the effect of telmisartan on endothelial progenitor cells (EPCs) function in patients with metabolic syndrome. Methods: 76 patients with metabolic syndrome were randomly divided into routine group and experimental group, 38 cases, while 38 healthy subjects were selected as the control group. The experimental group was treated with telmisartan 80 mg / d on the basis of conventional treatment. Peripheral blood was collected before treatment and 4 weeks after treatment. EPCs were isolated by density gradient centrifugation and identified by immunofluorescence double staining. Flow cytometry The proliferation and adhesion of EPCs were observed by adherence method for 30 min. The secretion of nitric oxide (NO) was detected by Griess method. The expression of endothelial nitric oxide synthase (eNOS) gene was detected by RTPCR semi-quantitatively. Results: Compared with the conventional group, EPCs in vitro showed significantly enhanced proliferation and adhesion in vitro, increased NO secretion and eNOS gene expression significantly (all P <0.05). Conclusion: Telmisartan can improve EPCs function in patients with metabolic syndrome, which is related to the up-regulation of eNOS gene expression and NO secretion.