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本文对缺血组织进行了水溶性莤素红(Alizarin Red S以下简称ARS)钙组织化学染色观察。此外还作了HE染色和心电图(ECG)检查。ARS染色阳性说明细胞内钙浓度异常增高。结果:正常对照组和缺血损伤组非缺血区对照ARS染色均为阴性;缺血区中,15分钟组9例动物中的1例和45分钟组12例动物中的7例为ARS阳性。上述各组的系列切片HE染色均未见坏死迹象。ECG检查发现8只动物有坏死性心电改变,出现异常Q波或QS波,恰与ARS(+)动物呈严格的对应关系。这些结果证明,ARS染色是一项敏感可靠的判定不可逆性缺血再灌注损伤的指标,它能够及时在细胞原位反映出钙增多这一致死性代谢改变。
In this paper, ischemic tissue was subjected to calcium histochemical staining of Alizarin Red S (ARS). In addition, he also made HE staining and electrocardiogram (ECG) examination. ARS staining positive abnormal intracellular calcium concentration. Results: The ARS staining in the non-ischemic area of the normal control group and the ischemic injury group were all negative. In the ischemic area, 1 of 9 animals in 15 minutes group and 7 of 12 animals in 45 minutes group were ARS positive . No signs of necrosis were seen in the HE slices of the above groups. ECG examination found that 8 animals have necrotic changes in ECG, abnormal Q waves or QS waves, just ARS (+) animals showed a strict correspondence. These results demonstrate that ARS staining is a sensitive and reliable indicator of irreversible ischemia-reperfusion injury that promptly reflects the lethal metabolic alteration of calcium in situ in the cells.