论文部分内容阅读
背景与目的:组织芯片(组织微阵列)是继基因芯片和蛋白质芯片之后出现的一种重要的生物芯片技术,是后基因组时代基因组学和蛋白质组学研究的重要方法之一。为了克服现有组织芯片仪器不够先进、操作繁琐、取样点样精度低等缺点,拟设计一种制作组织芯片的新方法。方法:在普通光学显微镜上加装特制的打孔针和取样针,在显微镜载物台上安置特制的石蜡块固定盒,利用显微镜精密的机械控制功能,进行受体石蜡块打孔和供体石蜡组织的取样、点样,并借助显微体视镜进行精确的取样部位观察与定位。结果:简化了组织芯片制作方法,使打孔、定位、取样和点样操作在同一仪器同一操作平台完成,并首次采取一次性使用的打孔针和取样针,提高了取样精度,避免了不同标本之间的组织残留和污染,成功制作了高质量的大肠肿瘤组织芯片。结论:改装普通显微镜用以制作组织芯片方法简便,技术可靠,费用低廉,值得推广。
BACKGROUND & OBJECTIVE: Tissue microarray (TGA) is an important biochip technique after gene chip and protein chip. It is one of the important methods of genomics and proteomics in the post-genome era. In order to overcome the shortcomings of the existing tissue chip instrument is not advanced enough, cumbersome operation, sample sampling accuracy is low, to design a new method of making tissue chip. Methods: A special perforated needle and a sampling needle were installed on the ordinary optical microscope. A special paraffin block fixation box was placed on the microscope stage. Using the precision mechanical control function of the microscope, paraffin blocks were punched and donated Paraffin tissue sampling, spotting, and microscopic endoscopy with accurate sampling site observation and positioning. Results: The method of making tissue chips was simplified. The drilling, positioning, sampling and spotting operations were performed on the same platform of the same instrument. For the first time, disposable perforating needles and sampling needles were used to improve the sampling precision and avoid the difference Tissue residue and contamination between the specimens, the successful production of high-quality large intestine tumor tissue chip. Conclusion: It is worth to popularize that it is simple, technically reliable, low cost to modify the common microscope to make tissue chip.