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作者用小鼠和豚鼠模型比较了磷酸铝(AIPO_4)、磷酸钙(CaHPO_4)和十八烷酰酪氨酸(ST)用作破伤风类毒素(TT)佐剂的活性.TT为三种不同纯度的制剂:(1)福马林脱毒、硫酸铵纯化,用于儿童和成人常规免疫接种的标准TT(AS-TT);(2)超滤去除低分子量肽的AS-TT(UF-TT);(3)硫酸铵纯化后再经Sephadex G-50凝胶过滤和二乙基氨基乙基柱层析,然后用福马林脱毒的层析纯化TT(CP-TT),分别制成吸附型和水溶型制剂.吸附剂PlPO_4、CaHPO_4和ST的浓度分别为4mg/ml、3.6mg/ml 和1mg/ml,TT水溶型制剂是直接溶于pH7.2的PBS制成,
The authors compared the activity of aluminum phosphate (AIPO_4), calcium phosphate (CaHPO_4) and stearyl tyrosine (ST) as adjuvants for tetanus toxoid (TT) using the mouse and guinea pig models.TT is a combination of three different Purity: (1) standard TT (AS-TT) for routine immunization of children and adults with dextromethorphan, ammonium sulfate purification; (2) ultrafiltration to remove low molecular weight peptides from AS-TT TT); (3) Purification of ammonium sulfate followed by Sephadex G-50 gel filtration and diethylaminoethyl column chromatography followed by purification of TT (CP-TT) The adsorbents PlPO_4, CaHPO_4 and ST were 4mg / ml, 3.6mg / ml and 1mg / ml respectively, and the TT water-soluble preparation was prepared by directly dissolving PBS in pH7.2,