目的建立一种以目视比色法半定量测定人尿液中锌含量的快速测定方法。方法在表面活性剂存在下,锌与硫氰酸盐和结晶紫形成三元离子缔合物,该胶束增溶体系随三元缔合物胶束的浓度不同而显示不同的颜色。结果经测定36份样本,与吡啶偶氮萘酚(PAN)显色法测定结果比较,两结果的符合率为97.22%。结论该方法简便、快速、结果准确直观,可用于尿液锌含量的半定量快速测定。 OBJECTIVE To establish a rapid method for the semi-quantitative determination of zinc in human urine by visual colorimetry. Methods In the presence of a surfactant, zinc forms a ternary ion association with thiocyanate and crystal violet. The micellar solubilization system shows different colors depending on the concentration of the ternary association micelles. Results 36 samples were determined. The coincidence rate of the two results was 97.22% compared with the results of the colorimetric determination of pyridylazonaphthol (PAN). Conclusion The method is simple, rapid, accurate and intuitive. It can be used for the rapid determination of zinc in urine.