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葡萄糖氧化酶系从微生物Aspergillusniger提取。它用于工业分析、医疗诊断以及酶电极的制备。为提高其热稳定性和对蛋白酶等的抗性,进行了葡萄糖氧化酶的修饰工作。牛血清白蛋白活性酯法曾用于小分子多肽的修饰,经适当改进后,应用于葡萄糖氧化酶的修饰。由于避免使用活泼的小分子交联剂(如戊二醛)取得了较满意的结果,酶活力回收率和结合率均接近100％。修饰葡萄糖氧化酶的性质研究,表明在实验条件下,每个葡萄糖氧化酶分子约连接2～3个牛血清白蛋白分子。由于后者的稳定作用和屏蔽作用,修饰酶的热稳定性以及抗
Glucose oxidase is extracted from the microorganism Aspergillus niger. It is used in industrial analysis, medical diagnostics and enzyme electrode preparation. In order to improve its thermal stability and protease resistance, the modification of glucose oxidase was carried out. Bovine serum albumin active ester method has been used for the modification of small molecule peptides, with appropriate improvements, applied to the modification of glucose oxidase. Due to the avoidance of the use of lively small molecule crosslinkers (eg, glutaraldehyde), satisfactory results have been obtained, with both the rate of recovery of the enzyme activity and the binding rate close to 100%. Studies on the properties of modified glucose oxidase indicate that about 2 to 3 bovine serum albumin molecules are attached per glucose oxidase molecule under experimental conditions. Due to the latter’s stabilizing effect and shielding effect, the thermostability and anti-enzyme modification enzymes are modified