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目的采用白蛋白纳米粒包载As2O3,通过肿瘤细胞摄取载药纳米粒来增强As2O3对K562细胞的增殖抑制作用。方法采用去溶剂化法制备白蛋白纳米粒(ALB-NP),以异硫氰酸(FITC)标记ALB-NP,荧光显微镜观察K562细胞对ALB-NP的摄取;以ALB-NP包载As2O3制备载As2O3白蛋白纳米粒(As2O3-ALB-NP),MTT法比较As2O3与As2O3-ALB-NP对K562细胞增殖抑制率的差异。结果 As2O3-ALB-NP在低浓度(<0.8μmol.L-1)即可显著抑制K562细胞增殖,而As2O3在该浓度对其无抑制作用。结论与As2O3相比,利用ALB-NP载As2O3可显著增强其对K562细胞的增殖抑制作用,有望实现对As2O3用药的增效减毒,为其用于抗肿瘤治疗提供了新的给药策略。
OBJECTIVE To investigate the inhibitory effect of As2O3 on proliferation of K562 cells by encapsulating As2O3 with albumin nanoparticles and taking drug-loaded nanoparticles by tumor cells. Methods The albumin nanoparticles (ALB-NP) were prepared by desolvation method. ALB-NP was labeled with FITC and the uptake of ALB-NP by K562 cells was observed by fluorescence microscopy. As2O3 albumin nanoparticles (As2O3-ALB-NP), MTT method compared As2O3 and As2O3-ALB-NP proliferation inhibition rate of K562 cells. Results As2O3-ALB-NP significantly inhibited the proliferation of K562 cells at low concentration (<0.8μmol.L-1), while As2O3 did not inhibit the proliferation of K562 cells at this concentration. Conclusion Compared with As2O3, As2O3 loaded ALB-NP can significantly enhance its inhibitory effect on proliferation of K562 cells. It is expected to achieve synergistic and attenuated effects on As2O3 and provide a new drug delivery strategy for anti-tumor therapy.