作者将伤寒杆菌纯培养物用磷酸盐缓冲液洗后分置在4个平皿内,其中3个置太阳光下照射杀菌,1个放在实验室工作台,每隔30分钟检测有无细菌生长。结果经阳光照射的平皿8小时后细菌被完全杀死,而实验室中的平皿上细菌依然存活。为比较阳光照射杀菌和丙酮杀菌制备的伤寒杆菌抗原的免疫原性,分别用阳光照射8、8.5、9小时的伤寒杆菌抗原和2倍、3倍、5倍体积丙酮杀菌的伤寒杆菌抗原免疫体重为180～250克的白化体小鼠,取眶后静脉血,用凝集试验检测伤寒抗体。 The author will be pure cultures of typhoid bacilli washed with phosphate buffer and then placed in 4 dishes, of which 3 placed under the sun irradiation sterilization, one on the laboratory bench, every 30 minutes to detect the presence of bacterial growth . Results The bacteria were completely killed 8 hours after exposure to the sun-exposed plate, while the bacteria on the plate in the laboratory remained viable. In order to compare the immunogenicity of typhoid bacillus antigen prepared by sunlight irradiation and acetone bactericidal treatment, the effects of typhoid bacilli antigen exposed to sunlight for 8, 8.5 and 9 hours and bactericidal Salmonella typhimurgens twice, 3 times and 5 times 180 to 250 grams of albino body mice, orbital venous blood taken by agglutination test typhoid antibodies.