采用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱及半制备高效液相色谱等方法对细叶石仙桃进行分离纯化,研究细叶石仙桃乙酸乙酯部位的化学成分,并运用核磁共振波谱法鉴定化合物的结构.结果表明,从细叶石仙桃乙酸乙酯部位分离得到的6种化合物分别是1,5-二羟基-2,7-二甲氧基-9,10-二氢菲(Ⅰ)、佛手柑内酯(Ⅱ)、甲氧基欧芹酚(Ⅲ)、异香草酸(Ⅳ)、阿魏酸二十六酯(Ⅴ)和二十四亚甲基环阿尔廷醇(Ⅵ),且化合物Ⅰ~Ⅴ为首次从该植物中分离得到. The silica gel column chromatography, ODS column chromatography, Sephadex LH-20 column chromatography and semi-preparative high performance liquid chromatography methods were used to separate and purify the leaves of Echinacea purpurea, The structures of the compounds were identified by spectroscopy.The results showed that the six compounds isolated from the ethyl acetate fraction of Picea meyeri respectively were 1,5-dihydroxy-2,7-dimethoxy-9,10-dihydrophenanthrene (Ⅰ), bergapten (Ⅱ), methoxy paeonol (Ⅲ), is vanillic acid (Ⅳ), hexadecyl ferulate (Ⅴ) Ⅵ), and compounds Ⅰ ~ Ⅴ were isolated from this plant for the first time.