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目的探讨贵州省博尔纳病病毒(BDV)的感染状况及其与神经系统疾病发生的相关性。方法建立检测外周血博尔纳病病毒的免疫印迹(Western blot,WB)方法,收集病毒性脑炎(VE)患者62例,帕金森病(PD)患者32例,格林-巴利综合征(GBS)患者19例,多发性硬化(MS)患者7例,以及体检中心健康人120例外周血标本中博尔纳病病毒核蛋白(P40)抗体,并对所检出的阳性标本进行确认。结果病例组阳性结果 8例(6.7%),其中病毒性脑炎患者4例,帕金森病患者2例,格林巴利综合征1例,多发性硬化1例。在所检测出的8例阳性标本中,病毒性脑炎患者阳性率为6.45%(4/62),帕金森病患者阳性率为6.25%(2/32),格林-巴利综合征患者阳性率为5.26%(1/19),多发性硬化患者阳性率为14.29%(1/7)。健康体检者对照组外周血中未检出阳性病例(0%)。神经系统疾病患者明显高于健康体检者(P=0.003,<0.05),有显著统计学差异,具有统计学意义。结论贵州省人群中存在博尔纳病病毒的感染,博尔纳病病毒的感染可能与神经系统疾病(脑炎等)的发生有更大的关系。
Objective To investigate the infection status of Borna disease virus (BDV) in Guizhou Province and its relationship with the occurrence of nervous system diseases. Methods Western blot (WB) was used to detect Borna disease in peripheral blood. Sixty-two patients with viral encephalitis (VE), 32 patients with Parkinson’s disease (PD) and 32 patients with Guillain-Barre syndrome 19 cases of GBS, 7 cases of multiple sclerosis (MS) and 120 cases of PBMC from healthy volunteers. The positive samples were confirmed. Results The positive results were 8 cases (6.7%) in the case group, including 4 cases of viral encephalitis, 2 cases of Parkinson’s disease, 1 case of Guillain-Barre syndrome and 1 case of multiple sclerosis. Of the 8 positive samples tested, the positive rate of viral encephalitis was 6.45% (4/62), the positive rate of Parkinson’s disease was 6.25% (2/32), the positive of Guillain-Barre syndrome The rate was 5.26% (1/19). The positive rate of multiple sclerosis patients was 14.29% (1/7). No positive cases were detected in the peripheral blood of healthy controls (0%). Patients with neurological diseases were significantly higher than healthy subjects (P = 0.003, <0.05), with significant statistical difference. Conclusion The infection of Borna disease virus among Guizhou population may be related. The infection of Borna disease virus may have a greater relationship with the occurrence of nervous system diseases (encephalitis, etc.).