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目的采用胶束毛细管电泳在线推扫法测定中成药中的微量士的宁。方法运行缓冲液为50 mmol.L-1NaH2PO4-90 mmol.L-1SDS-乙腈(83∶83∶34),pH2;0.5 psi压力进样,进样时间3.7 min;分离电压25 kV;分离温度25℃;检测波长205 nm。结果士的宁0.5～8.0μg.mL-1峰面积线性关系良好(r=0.9998),检测限达0.020μg.mL-1;方法回收率>97.6%;日内、日间RSD均小于1.8%。结论所用方法快速、准确,有效提高了毛细管电泳法的灵敏度。
Objective To determine the amount of trace amount of strychnine in proprietary Chinese medicines by micellar capillary electrophoresis online push-sweep method. The running buffer was 50 mmol.L-1NaH2PO4-90 mmol.L-1SDS-acetonitrile (83:83:34), pH2; 0.5 psi pressure injection, injection time was 3.7 min; separation voltage was 25 kV; separation temperature was 25 ℃; detection wavelength 205 nm. Results The calibration curve of strychnine 0.5 ~ 8.0μg.mL-1 was linear (r = 0.9998). The detection limit was 0.020μg.mL-1. The recovery rate was 97.6%. The intra-day and interday RSD were less than 1.8%. Conclusion The method used is rapid, accurate and effective to improve the sensitivity of capillary electrophoresis.