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考察了填充柱超临界流体色谱法 (SFC)中的样品溶剂及连续进样等因素对化合物保留行为变化的影响规律。以超临界 CO2 或含低体积分数甲醇的 CO2 为流动相时 ,氨基柱上组分的保留时间随着样品溶剂的极性增大而增大 ,而溶剂对 C1 8柱上组分的保留时间影响不大 ;在 C1 8柱上 ,溶剂对连续进样的后续效应不强 ;而在氨基柱上 ,甲醇溶液的后续效应比丙酮、氯仿溶液的后续效应强。当甲醇的体积分数大于 1 .0 %时 ,溶剂的效应明显减弱。这种变化规律对填充柱 SFC的合理进样并获得重现性良好的色谱数据具有实际意义。
The effects of sample solvent and continuous injection on the retention behavior of the compounds were investigated in packed-column supercritical fluid chromatography (SFC). When using supercritical CO2 or CO2 with low volume fraction of methanol as the mobile phase, the retention time of the components on the amino column increases with the increase of the polarity of the sample solvent, while the retention time of the components on the C1 8 column The effect on continuous injection was not strong on the C1 8 column, but the follow-up effect of methanol solution was stronger than that of acetone and chloroform solution on the amino column. When the volume fraction of methanol is greater than 1.0%, the solvent effect is significantly reduced. This variation has practical implications for proper sampling of packed SFCs and good reproducibility of chromatographic data.