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脂肪过度沉积是引起肥胖的最主要原因。脂肪沉积水平受到脂肪代谢的影响。CIDEC是近年研究新发现的一种脂肪滴结合蛋白,是调控脂肪滴发育和脂肪沉积的关键因子。本研究以小鼠为动物模型,采用Co-IP和质谱分析等实验方法,从蛋白功能、亚细胞定位、GO、KEGG功能及其富集度等方面解析CIDEC在高脂和禁食条件下互作蛋白的差异,为理解CIDEC感应机体能量状态的分子机制,及在不同生理条件下的信号传导和功能转换提供实验依据。
Over-deposition of fat is the most important cause of obesity. The level of fat deposition is affected by fat metabolism. CIDEC is a fat-drop protein newly discovered in recent years and is a key factor in regulating fat drop development and fat deposition. In this study, mice were used as animal models to analyze the functions of CIDEC in high fat and fasting conditions from the aspects of protein function, subcellular localization, GO, KEGG function and their enrichment by using the methods of Co-IP and mass spectrometry Differences in protein content provide an experimental basis for understanding the molecular mechanism of CIDEC sensing of energy status and the signal transduction and function switching under different physiological conditions.