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目的:为了了解胃癌的生物学行为.方法:采用DNA/角蛋白双参数流式细胞术测定50例胃癌细胞DNA含量、S期及G_2/M期细胞比率,并就其与胃癌生物学特性进行分析.结果:二倍体癌,浆膜受侵者S期细胞比率明显高于未受侵者(P<0.05).异倍体癌,浆膜受侵者G_2/M期细胞比率明显高于未受侵者(P<0.05),团块状生长者G_2/M期细胞比率明显高于弥漫状生长者(P<0.05),淋巴结转移阳性者G_2,/M加期细胞比率明显高于阴性者(P<0.05),淋巴结转移枚数多于5枚者的S和G_2/M期细胞比率分别明显高于淋巴结转移阴性和转移枚数少于5枚者(P<0.05),淋巴结转移至第Ⅱ站以远者G_2/M期细胞比率明显高于淋巴结转移阴性和转移至第Ⅰ站者(P<0.05).结论:胃癌细胞DNA含量和细胞增殖比率与胃癌生物学行为确有一定关系.
Objective: To understand the biological behavior of gastric cancer. METHODS: DNA, keratin, and two-parameter flow cytometry were used to determine DNA content, S-phase and G2/M phase cell ratios in 50 gastric cancer cells, and their biological characteristics with gastric cancer were studied. Analysis. Results: In diploid cancer, the ratio of S-phase cells in serosa invaded persons was significantly higher than that in uninvaded patients (P<0.05). The ratio of G2/M phase cells in serosa invaded patients was significantly higher than in diploid carcinomas. In the uninvaded group (P<0.05), the ratio of G2/M phase cells was significantly higher in mass growth than in those with diffuse growth (P<0.05). The ratio of G_2, M cells in positive phase of lymph node metastasis was significantly higher than that in negative masses. Among the patients (P<0.05), the ratios of S and G2/M phase cells with more than 5 lymph node metastases were significantly higher than those with negative lymph node metastasis and fewer than 5 metastases (P<0.05). Lymph node metastasis to II The percentage of G2/M phase cells that was beyond the station was significantly higher than that of negative lymph node metastasis and metastasis to the first station (P<0.05). Conclusions: The DNA content and cell proliferation ratio of gastric cancer cells have a certain relationship with the biological behavior of gastric cancer.