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目的建立急性心肌梗死(AMI)致应激性高血糖大鼠的实验动物模型。方法 40只SD雄性大鼠随机均分为四组。A、B、C组结扎左冠状动脉前降支,分别于缺血15、30和45min后再灌注120min;S组为假手术对照。持续监测各组心电图和缺血前(T0)、缺血末(T1)和再灌注末(T2)时的血糖,检测T2时血促肾上腺皮质激素(ACTH)、皮质酮和胰岛素水平,2,3,5-氯化三苯基四氮唑(TTC)染色分析心肌梗死面积,观察模型成功及大鼠死亡的比例。结果 A、B、C组大鼠结扎前降支后均出现了显著的ST-T改变,再灌注后可见病理性Q波,TTC染色出现白色梗死区。与S组比较,A、B、C组T1、T2时的血糖和T2时的ACTH、皮质酮、胰岛素水平均升高(P<0.05),且B、C组高于A组(P<0.05)。B、C组模型成功比例高于A组(P<0.05),B组和C组分别有1只和3只大鼠死亡。结论成功建立AMI致应激性高血糖实验动物模型;缺血30min再灌注120min的模型稳定,病死率低。
Objective To establish an experimental animal model of acute hyperglycemia induced by acute myocardial infarction (AMI) in rats. Methods 40 SD male rats were randomly divided into four groups. A, B, C group ligation of left anterior descending coronary artery, respectively, at 15, 30 and 45min after ischemia reperfusion 120min; S group was sham control. The electrocardiogram (ECG) and the blood glucose before T 0, T 1 and T 2 were monitored continuously. The levels of ACTH, corticosterone and insulin in T2 were measured. The area of myocardial infarction was analyzed by 3, 5-TTC staining. The success rate of model and the proportion of death in rats were observed. Results In group A, B and C, significant ST-T changes occurred in all rats before descending coronary artery ligation. Pathological Q wave was observed after reperfusion, and white infarction zone was found on TTC staining. Compared with group S, blood glucose at T1, T2 and levels of ACTH, corticosterone and insulin at T2 were significantly higher in groups A, B and C (P <0.05), and in groups B and C were higher than those in group A (P <0.05 ). The success rates of group B and group C were higher than those of group A (P <0.05), and those of group B and C were 1 and 3 respectively. Conclusion The animal models of acute hyperglycemia induced by AMI were successfully established. The models of ischemia-reperfusion 120 min after ischemia 30 min were stable and the mortality was low.