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目的:研究胆囊胆固醇结石患者肝脏的核受体基因:肝脏x受体a(1iver X receptor a,LXRα)、法尼醇受体(farnesoid Xreceptor,FXR)、人类固醇异生物受体(steroid xenobiotic receptor,SXR)及肝受体同类物1(1iver receptor homolog-1,LRH-1)的蛋白表达,探讨胆固醇结石病的发病机理。方法:23例胆囊胆固醇结石患者(胆石组),12例无胆石症的胆囊息肉患者为对照(对照组)。测定胆石胆固醇成分和胆汁脂类成分(胆固醇、磷脂和胆汁酸),并计算胆汁总脂和胆汁胆固醇饱和指数。Western-Blot法测定肝脏LRH-1、FXR、SXR及LXRa基因的蛋白表达量。结果:胆石组胆汁呈胆固醇过饱和;胆汁胆固醇摩尔百分比浓度较对照组升高,P<0.01;胆汁总脂较对照组明显下降,P<0.05;胆汁中胆汁酸和磷脂成分2组比较差异均无统计学意义(P>0.05)。胆石组LRH-1蛋白表达高于对照组(0.88±0.05vs 0.69±0.03),P<0.05,LXRa、FXR和SXR表达2组差异无统计学意义(P>0.05)。结论:人类肝脏LRH-1的蛋白表达增高与胆囊胆固醇结石形成有关
OBJECTIVE: To study the expression of nuclear receptor genes in liver of patients with gallstone gallstone: liver 1 receptor X receptor a (LXRα), farnesoid X receptor (FXR), steroid xenobiotic receptor , SXR) and liver receptor homolog-1 (LRH-1) protein expression, to explore the pathogenesis of cholesterol gallstone disease. Methods: Twenty-three patients with gallstone gallstone (gallstone group) and 12 patients with gallstone polyp without cholelithiasis (control group). Cholesterol components and bile lipids (cholesterol, phospholipids and bile acids) were determined and total bile fat and bile cholesterol saturation indices were calculated. The protein expression of LRH-1, FXR, SXR and LXRa in liver were measured by Western-Blot. Results: The bile in gallstone group was hypercholesterolemic; the molar concentration of bile cholesterol was higher than that in control group (P <0.01); the total cholesterol in bile group was significantly lower than that in control group (P <0.05); bile acid and phospholipid No statistical significance (P> 0.05). The expression of LRH-1 in gallstone group was significantly higher than that in control group (0.88 ± 0.05 vs 0.69 ± 0.03, P <0.05). There was no significant difference in LXRa, FXR and SXR expression between the two groups (P> 0.05). Conclusions: The increased expression of LRH-1 in human liver is associated with gallstone gallstone formation