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目的探讨凋亡相关蛋白Fas、Bcl-2在胎盘滋养叶细胞中的表达及其意义,滋养叶细胞凋亡与Fas、Bcl-2两种蛋白的关系,从而于细胞凋亡的角度探讨子痫前期的发病机制。方法用免疫组织化学SP法检测重度子痫前期患者20例,轻度子痫前期20例(试验组),同时选择同期正常晚孕妇女20例(对照组)做胎盘组织中Fas、Bcl-2蛋白的表达及滋养叶细胞凋亡检测。结果细胞凋亡相关蛋白Fas、Bcl-2在子痫前期及正常孕妇胎盘组织中均有表达。Fas蛋白的表达在试验组明显高于对照组(P﹤0.05),Bcl-2蛋白的表达在试验组低于对照组(P﹤0.05);在试验组及对照组均可观察到细胞凋亡现象,在滋养层细胞(合体细胞滋养层及细胞滋养层)、间质细胞、血管内皮细胞胞浆及细胞核内均有表达,尤以合体细胞最多见。试验组细胞凋亡较对照组明显增多(P﹤0.05)。子痫前期时,Bcl-2与Fas蛋白的表达呈负性相关;Bcl-2蛋白表达与胎盘细胞凋亡呈负性相关,而Fas蛋白的表达与胎盘细胞凋亡呈正性相关(P﹤0.01)。结论子痫前期的发病原因可能与胎盘滋养叶细胞凋亡及Bcl-2、Fas基因异常表达有关。
Objective To investigate the expression and significance of Fas and Bcl-2 in placental trophoblast cells and the relationship between the apoptosis of trophoblast cells and the expression of Fas and Bcl-2 proteins, and to explore the relationship between apoptosis and apoptosis Early pathogenesis. Methods 20 cases of severe preeclampsia and 20 cases of mild preeclampsia (test group) were detected by immunohistochemical SP method. Meanwhile, 20 normal pregnant women (control group) Protein Expression and Trophoblast Cell Apoptosis Detection. Results The apoptosis-related proteins Fas and Bcl-2 were expressed in placenta of preeclampsia and normal pregnant women. The expression of Fas protein in the experimental group was significantly higher than that in the control group (P <0.05), and the expression of Bcl-2 protein was lower in the experimental group than in the control group (P <0.05). Apoptosis was observed in the experimental and control groups Phenomenon, in trophoblast cells (syncytiotrophoblast and cytotrophoblast), interstitial cells, vascular endothelial cells in the cytoplasm and nucleus are expressed, especially in the most common syncytial cells. The apoptosis in experimental group was significantly higher than that in control group (P <0.05). The expression of Bcl-2 protein was negatively correlated with the expression of Fas protein in preeclampsia, the expression of Bcl-2 protein was negatively correlated with the apoptosis of placental cells, and the expression of Fas protein was positively correlated with the apoptosis of placental cells (P <0.01 ). Conclusion The pathogenesis of preeclampsia may be related to the apoptosis of placental trophoblast cells and the abnormal expression of Bcl-2 and Fas genes.