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目的：主要探索Ｔ－２毒素对体外培养软骨细胞致损临界值以及硒保护作用。方法：应用单层培养传代软骨细胞，用生化方法检测软骨细胞ＤＮＡ量和蛋白量，透射电子显微镜观察软骨细胞超微结构。结果：（１）Ｔ－２毒素在０．００１～０．００５ｍｇ／Ｌ培养液中（以下相同）剂量范围内，随浓度增加，Ｔ－２毒素对软骨细胞ＤＮＡ合成和细胞分裂增殖影响逐渐增大，加硒后该影响有所减弱，但仍不能阻止损伤的发生；（２）Ｔ－２毒素在０．０００５ｍｇ／Ｌ时，对软骨细胞的影响甚微；Ｔ－２毒素在０．００１ｍｇ／Ｌ和０．００５ｍｇ／Ｌ时，细胞损伤明显，与对照组比较有显著差别（Ｐ＜０．００１）。
OBJECTIVE: To explore the critical value of T-2 toxin in vitro and the protective effect of selenium on chondrocytes. Methods: Chondrocytes were passaged in monolayer culture, DNA content and protein content of chondrocytes were detected by biochemical methods, and the ultrastructure of chondrocytes was observed by transmission electron microscopy. Results: (1) T-2 toxin gradually increased with increasing concentration of DNA in the range of 0.001-0.005 mg / L (same below) in T-2 toxin production and cell division in chondrocytes The effect was weakened after large selenium addition, but still could not prevent the occurrence of damage. (2) The effect of T-2 toxin at 0.0005 mg / L had little effect on chondrocytes; T-2 toxin at 0.001 mg / L and 0.005mg / L, the cell damage was significant compared with the control group there was a significant difference (P <0.001).