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目的测定乙型肝炎病毒(HBV)的医院感染阈值。方法在确定HBV携带者的感染剂量、最大传染性和传染所需最小血量的基础上,使用血液系列稀释法测定HBV的医院感染阈值。结果 HBsAg阳性和阴性、HBeAg阳性和阴性者的最大传染性分别是109、104、109、106ID/ml,传染所需的最小血量分别是10-9、10-4、10-9、10-6ml;HBV经单层手套传播的感染阈值为105ID/ml,经双层手套传播的防护阈值为106ID/ml,HBV感染阈值随接种血量的增加而减低,HBV浓度随感染阈值的升高而升高。结论感染阈值包含了病毒浓度和入体血量两方面的内容,与前者呈正相关,与后者呈负相关,在选择正确的传染源替代指标、制定合理的监控方法方面有实用价值。
Objective To determine the nosocomial infection threshold of Hepatitis B virus (HBV). Methods Based on the determination of the infection dose, the maximum infectivity and the minimum blood transfusion required for HBV carriers, the threshold of nosocomial infection of HBV was determined by serial dilution of blood. Results HBsAg positive and negative, HBeAg positive and negative were the maximum contagion were 109,104,109,106 ID / ml, the minimum amount of blood required for infection were 10-9,10-4,10-9,10- 6ml. The infection threshold of HBV transmitted by monolayer glove was 105ID / ml. The protection threshold of HBV transmitted by double glove was 106ID / ml. The threshold of HBV infection decreased with the increase of inoculation volume. The HBV concentration increased with the increase of infection threshold Rise. Conclusion The infection threshold contains both the concentration of the virus and the volume of the incoming blood, which is positively correlated with the former and negatively correlated with the latter. It is of practical value in selecting proper indicators of infection source substitution and formulating reasonable monitoring methods.