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目的分析江西省2009年-2012年B型流感病毒耐药基因位点,掌握其耐药情况,为临床治疗和疾病控制提供参考。方法采集监测医院和疑似流感疫情的流感样病例鼻咽拭子标本进行流感病毒分离,随机选择分离到的B型流感病毒进行核酸提取,采用逆转录-聚合酶链反应(RT-PCR)扩增病毒NA基因后进行核苷酸序列测定,用DNAStar5.0,Mage 3.1生物软件对测序结果进行分析处理,推导出氨基酸序列,进行基因特性分析。结果 24株B型流感病毒NA区域核苷酸序列长度均为1 398 bp,编码466个氨基酸,所有毒株的NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论 24株毒株均对流感病毒神经氨酸酶抑制剂药物敏感,但仍应加强对流感病毒的耐药性监测。
Objective To analyze the resistance gene loci of influenza B virus from 2009 to 2012 in Jiangxi Province and to master the drug resistance of the virus, providing reference for clinical treatment and disease control. Methods Nasopharyngeal swab specimens were collected from influenza-like cases in hospitals and suspected influenza epidemic for influenza virus isolation. The isolated influenza B viruses were selected for nucleic acid extraction at random and amplified by reverse transcription-polymerase chain reaction (RT-PCR) The nucleotide sequence of the virus NA gene was determined, and the sequencing results were analyzed by using DNAStar5.0 and Mage 3.1 biological software to deduce the amino acid sequence and analyze the gene characteristics. Results The nucleotide sequences of NA region of all the 24 influenza B viruses were 1 398 bp in length and encoded 466 amino acids. No amino acid substitutions were found in the catalytic and accessory sites of all the NA strains. Conclusion All 24 strains are sensitive to influenza virus neuraminidase inhibitors, but the surveillance of influenza virus resistance should be strengthened.