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本文报告用鞣化载体酶联 A 蛋白的“ELISA”法检测 DNA 抗体的研究。以OD 值492nm>0.14为阳性,对60例正常人和104例自身免疫病、心血管病及非免疫性疾病患者的1:25倍稀释血清进行了检测。上述同样标本也用免疫荧光法检测进行比较。酶标法阳性率为69.1%,荧光法阳性率为47.6%,提示酶标法比荧光法有较高的敏感性。本文也对其影响因素进行了探讨。结果显示该法对检测抗核抗体是一种简便、快速、特异、敏感的方法,其 OD 值的测定又具有定性与定量的双重
This article reports the use of tanning of the enzyme-linked protein A ELISA test method for the detection of DNA antibodies. The OD value of 492nm> 0.14 as a positive, 60 cases of normal subjects and 104 cases of autoimmune diseases, cardiovascular disease and non-immune disease in patients with 1:25 dilution of serum were detected. The same samples were also compared by immunofluorescence assay. The positive rate of enzyme-labeled method was 69.1%, the positive rate of fluorescence method was 47.6%, suggesting that enzyme-labeled method had higher sensitivity than fluorescence method. This article also discusses its influencing factors. The results show that the method for the detection of anti-nuclear antibody is a simple, rapid, specific and sensitive method, the determination of OD value has qualitative and quantitative double