试管快速繁殖葡萄苗时,首先要获得无菌材料,我们通过7年的实践,找出了一套较好的葡萄茎段消毒方法,介绍如下:当地生长的嫩梢消毒从植株上部剪取嫩梢,立即拿回室内,剪除叶片,装入广口瓶内,用不同的方法消毒。京早晶品种用酒精棉球擦嫩梢后,再用0.1%升汞消毒15分钟,污染率为3.6%,受害率为32.1%。用70%酒精浸5秒钟,加0.1%的升汞消毒15分钟,污染率为10%,受害率为35%。若用0.1%升汞消毒12分钟,污染率为14.8%。用2%酒精+0.1%升汞消毒12分钟,污染率10%。用2%酒精+5%次氯酸钠消毒15分钟,污染率为60%。以上 Test tube rapid propagation of grape seedlings, we must first get aseptic material, we passed 7 years of practice, we found a good method of grape stem disinfection, introduced as follows: local shoots of young shoots from the upper plant cut Shoot, immediately get back indoors, cut off the leaves, into jars, disinfected in different ways. Jing Zaojing varieties wipe the tips with alcohol cotton balls, and then disinfected with 0.1% mercuric chloride for 15 minutes, the pollution rate was 3.6%, the damage rate was 32.1%. Baptist with 70% alcohol for 5 seconds, plus 0.1% mercury chloride disinfection for 15 minutes, the pollution rate was 10%, the victimization rate was 35%. If disinfected with 0.1% mercuric chloride for 12 minutes, the contamination rate is 14.8%. Disinfection with 2% alcohol + 0.1% mercuric chloride for 12 minutes, the pollution rate of 10%. Disinfect 15% with 2% alcohol + 5% sodium hypochlorite at a contamination rate of 60%. the above