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目的:探讨苦参碱对T淋巴细胞增殖和细胞因子IL-2、IFN-γ的影响。方法:使用苦参碱在不同浓度或在不同时间内对人T淋巴细胞进行体外诱导培养,利用CCK-8法检测不同浓度苦参碱对T淋巴细胞增殖的影响程度。用ELISA试剂盒测定培养上清液中的IL-2、IFN-γ细胞因子水平。结果:苦参碱在0.1、0.5mg/mL剂量范围内能促进T淋巴细胞增殖反应,而1.0、2.0和3.0mg/mL苦参碱对人T淋巴细胞增殖呈抑制作用。人T淋巴细胞受不同浓度苦参碱刺激时,0.1、0.5mg/mL苦参碱组IL-2和IFN-γ水平与对照组相比显著升高(P<0.01),1.0mg/mL苦参碱抑制IL-2和IFN-γ的分泌。结论:苦参碱对人外周血T淋巴细胞的增殖呈双向作用;低浓度苦参碱能诱导人T淋巴细胞分泌IL-2和IFN-γ。
Objective: To investigate the effects of matrine on T lymphocyte proliferation and cytokines IL-2 and IFN-γ. METHODS: Human T lymphocytes were cultured in vitro at different concentrations or at different time points using matrine. The effects of different concentrations of matrine on the proliferation of T lymphocytes were detected by CCK-8. The levels of IL-2 and IFN-γ cytokines in culture supernatants were determined by ELISA kit. Results: Matrine could promote the proliferation of T lymphocytes in the dose range of 0.1 and 0.5 mg / mL, while matrine at 1.0, 2.0 and 3.0 mg / mL could inhibit the proliferation of human T lymphocytes. Compared with the control group, the levels of IL-2 and IFN-γ in 0.1, 0.5mg / mL matrine group were significantly increased (P <0.01) and 1.0mg / mL Ginseng inhibits the secretion of IL-2 and IFN-γ. Conclusion: Matrine has a bidirectional effect on the proliferation of human peripheral blood T lymphocytes. Low concentrations of matrine can induce the secretion of IL-2 and IFN-γ by human T lymphocytes.