论文部分内容阅读
目的 :观察辛伐他汀对高胆固醇血症小鼠离体胸主动脉内皮粘附单核细胞的抑制效应。方法 :用不同剂量 (1,5 ,2 0mg·kg- 1)的辛伐他汀每日1次给高胆固醇血症小鼠皮下注射共 2wk或 6wk ,届时剪取小鼠胸主动脉 ,用于单核细胞粘附试验。结果 :(1)只有 2 0mg·kg- 1× 6wk组小鼠血浆胆固醇水平显著降低 ,从 6 .6± 0 .7mmol·L- 1降为 4 .6± 0 .7mmol·L- 1,(P <0 .0 5 ) ;(2 )高胆固醇血症小鼠胸主动脉内皮对单核细胞的粘附率明显高于正常小鼠 ,辛伐他汀 2 0mg·kg- 12个组对高胆固醇血症小鼠胸主动脉内皮粘附单核细胞有明显的抑制作用 ,粘附率 (4.1± 2 .5 ) %vs (1.9± 0 .1) % (和 )或(1.4± 0 .3) %均 (P <0 .0 5 )。结论 :高胆固醇血症小鼠胸主动脉内皮对单核细胞粘附增强 ,辛伐他汀对高胆固醇血症小鼠血管内皮粘附单核细胞有明显的抑制作用
OBJECTIVE: To observe the inhibitory effect of simvastatin on the adhesion of monocytes isolated from the thoracic aorta in hypercholesterolemic mice. Methods: Hypercholesterolemic mice were injected subcutaneously with simvastatin at different doses (1,5, 20 mg · kg-1) once a day for 2 or 6 weeks, then the thoracic aorta was excised for Monocyte adhesion test. Results: (1) The plasma cholesterol level of 20 mg · kg-1 × 6wk mice decreased significantly from6.6 ± 0.7mmol·L-1to4.6 ± 0.7mmol·L-1, ( P <0.05); (2) The adhesion rate of thoracic aorta to monocytes in hypercholesterolemic mice was significantly higher than that in normal mice. Simvastatin 20 mg · kg- The hyperlipidemic mice had obvious inhibitory effect on the adhesion of monocytes in the thoracic aorta. The adhesion rate was (4.1 ± 2.5)% vs (1.9 ± 0.1)% and (1.4 ± 0.3) % (P <0.05). Conclusion: The hypercholesterolemic mouse thoracic aorta adhesion to monocytes enhanced, simvastatin on vascular hypercholesterolemia adhesion of monocytes in endothelial cells was significantly inhibited