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目的:观察人参皂甙-Rd(Ginsenoside-Rd,G-Rd)对坐骨神经分支选择损伤(spared nerve injury,SNI)大鼠痛敏异常及脊髓背角内P物质(substance P,SP)和NK-1受体表达的影响,进而探讨G-Rd镇痛的脊髓机制。方法:成年雄性SD大鼠(30只)随机分成五组:空白对照组(blank control)、假手术组(sham operation)、坐骨神经分支选择损伤组(spared nerve injury,SNI)、SNI+saline(腹腔注射,i.p.)组、SNI+G-Rd(i.p.)组。行为学采用von Frey法测定上述各组手术侧机械缩足反射阈值(paw withdrawal mechanical thresholds,PWMT);用免疫荧光组织化学染色法检测对比上述各组大鼠脊髓L4-6节段背角内SP免疫荧光产物的荧光强度和NK-1阳性细胞的数量。结果:SNI术后10 d,大鼠手术侧PWMT值明显低于正常对照组和假手术组,术后20 d到达最低值;而在SNI+G-Rd组PWMT值则明显高于SNI组和SNI+sline组(P<0.05)。免疫荧光染色结果显示:术后20 d时,SNI组和SNI+saline组的脊髓手术侧L4-6背角内SP样免疫荧光的强度和NK-1样阳性细胞的数量明显有所增高,与对照组和假手术组相比较具有显著性意义(P<0.05);但SNI+G-Rd组与SNI组和SNI+saline组相比,其SP样免疫荧光的强度和NK-1样阳性细胞的数量则明显有所下降(P<0.05)。结论:人参皂甙-Rd抑制神经病理性疼痛的机制之一可能与有效减少脊髓背角内SP和NK-1受体的表达有关。
Objective: To observe the effects of ginsenoside-Rd (G-Rd) on pain-related hyperalgesia and substance P (SP) and NK-1 in spinal dorsal horn in spared nerve injury (SNI) Receptor expression, and then explore the G-Rd analgesic spinal cord mechanism. Methods: Adult male SD rats (30 rats) were randomly divided into five groups: blank control group, sham operation group, spared nerve injury group (SNI), SNI + saline group Injection, ip) group, SNI + G-Rd (ip) group. Behavioral von Frey method was used to measure the paw withdrawal mechanical thresholds (PWMT) of the above groups. Immunofluorescence histochemical staining was used to detect and compare the spinal dorsal SP Fluorescence intensity of immunofluorescent products and the number of NK-1 positive cells. Results: On the 10th day after SNI, the value of PWMT in the operation side of the rats was significantly lower than that of the normal control group and the sham operation group, and reached the lowest value 20 days after the SNI operation. The PWMT value of the SNI + G-Rd group was significantly higher than that of the SNI group and SNI + sline group (P <0.05). The results of immunofluorescence staining showed that the intensity of SP immunofluorescence and the number of NK-1 positive cells in the L4-6 dorsal horn of the SNI group and the SNI + saline group were significantly increased at 20 d after operation Compared with sham-operation group and SNI + G-Rd group, the intensity of SP-like immunofluorescence and the expression of NK-1 positive cells in SNI group and SNI + saline group were significantly higher than those in sham operation group (P <0.05) The number was significantly decreased (P <0.05). CONCLUSION: One of the mechanisms by which ginsenoside-Rd inhibits neuropathic pain may be related to the effective reduction of SP and NK-1 receptor expression in spinal dorsal horn.