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目的:建立一种测定大鼠血浆中叶黄素的反相高效液相色谱法,研究大鼠灌胃叶黄素后的药动学特征。方法:血浆样品用二氯甲烷进行萃取,反相高效液相色谱法测定血浆中叶黄素浓度。色谱柱为ODS Hypersil C_(18)柱,流动相为乙腈-甲醇-二氯甲烷(60:20:20),流速0.7 mL·min~(-1),检测波长445 nm。SD大鼠单剂量灌胃给予10 mg·kg~(-1)叶黄素,分别测定给药后不同时间点血浆中药物的浓度并绘制药时曲线,计算药动学参数。结果:所得HPLC方法专属性较高,叶黄素在0.05～4μg·mL~(-1)范围内线性关系良好(r=0.998 9),精密度及加样回收率均符合要求。大鼠灌胃叶黄素混悬液3 h后达到血药浓度峰值140.81 ng·mL~(-1),药时曲线下面积为1 852.94 ng·h·mL~(-1),生物半衰期为25.25 h。结论:该方法简便可靠,能够满足血浆中叶黄素测定及药动学研究的要求。药动学结果显示,叶黄素经灌胃给药后在大鼠体内吸收速度较慢,吸收程度较低且消除缓慢。
Objective: To establish a RP-HPLC method for the determination of lutein in rat plasma and to study the pharmacokinetics of lutein in rats. Methods: Plasma samples were extracted with dichloromethane and the plasma concentrations of lutein were determined by RP-HPLC. The column was ODS Hypersil C_ (18) with a mobile phase of acetonitrile-methanol-methylene chloride (60:20:20) at a flow rate of 0.7 mL · min -1 with a detection wavelength of 445 nm. Sprague-Dawley rats were given a single dose of 10 mg · kg -1 of lutein, and the plasma concentrations of the drugs were measured at different time points after administration. The pharmacokinetic parameters were calculated. Results: The obtained HPLC method was of high specificity. Lutein had a good linearity (r = 0.998 9) in the range of 0.05-4μg · mL -1. The precision and sample recovery were satisfactory. After 3 h of intragastric administration of lutein in rats, the peak plasma concentration reached 140.81 ng · mL -1, and the area under the curve was 1 852.94 ng · h · mL -1. The half-life of the mixture was 25.25 h. Conclusion: The method is simple and reliable and can meet the requirements of lutein determination and pharmacokinetics in plasma. Pharmacokinetic results show that lutein after gavage administration in vivo absorption slower, less absorbed and slow elimination.