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目的观察苏云金杆菌CryIVD转基因贮存藻对不同龄期淡色库蚊幼虫的杀灭效果。方法大量培养工程藻,干燥后保存,应用不同时间的贮存藻作用于不同龄期淡色库蚊幼虫。结果干燥贮存的工程藻数年后仍有较高的杀蚊毒效,尽管杀蚊毒效较新鲜工程藻有所下降,但在105个/ml数量级不同龄期的幼虫仍有50%以上死亡。对不同龄期淡色库蚊幼虫杀灭效果比较表明,1~3年贮存藻3组比较,在106个/ml数量级浓度的蚊幼虫死亡率差异无统计学意义(F=9.32,P>0.01),而贮存1年组和3年组在105个/ml数量级浓度及以下,死亡率差异有统计学意义(F=101.21,P<0.01)。结论转基因蓝藻在干燥贮存情况下仍能保持毒蛋白的稳定遗传和杀幼虫作用,为今后的现场应用提供更方便的选择。
Objective To observe the killing effect of Bacillus thuringiensis CryIVD transgenic stocking on larvae of Culex pipiens pallens at different ages. Methods A large number of engineering algae were cultured and stored after drying. The stored algae of different ages were used to effect the larvae of Culex pipiens pallens at different ages. Results The engineering algae still had higher mosquito toxicity after several years of storage. Although the mosquito toxicity was lower than that of the fresh engineering algae, more than 50% of the larvae were still at the level of 105 / ml . Comparison of killing effects of Culex pipiens pallens larvae at different ages showed that there was no significant difference in the mortality of mosquito larvae at the concentration of 106 / ml between 1 and 3 years (F = 9.32, P> 0.01) , While there was a statistically significant difference in the mortality rates between the 1-year and 3-year groups at a concentration of 105 / ml (F = 101.21, P <0.01). Conclusion Transgenic cyanobacteria can still maintain stable heredity and larvicidal activity of toxic protein under the condition of dry storage, and provide a more convenient choice for future field application.