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目的探讨Beclin1和p62在结肠癌组织中和正常结肠黏膜的表达差异以及临床意义。方法采用免疫组织化学法对42例结肠患者癌组织以及正常黏膜组织中Beclin1以及p62的阳性表达率进行检测。比较不同病理资料中Beclin1以及p62的表达差异,并随访5年生存率,Cox回归分析影响结肠癌患者死亡的危险因素。运用SPSS 20.0统计软件进行统计学分析,P<0.05为差异有统计学意义。结果 Beclin1在正常肠黏膜中表达阳性率高于癌组织,另外在T1~T2期中的阳性表达率高于T3~T4期的阳性率,以上差异有统计学意义(P<0.05)。p62蛋白在癌组织中表达升高,在正常组织中表达降低,2组间差异有统计学意义(P<0.05)。Cox回归分析显示,对于结肠癌的患者,p62是患者死亡的危险因素,联合使用p62以及Beclin1是预测结肠癌预后的良好指标。结论结肠癌患者的自噬活性降低,Beclin1与CEA的表达水平以及肿瘤的浸润深度有关,联合应用Beclin1以及p62可以较好地预测结肠癌患者的预后。
Objective To investigate the differences and clinical significance of Beclin1 and p62 expression in colon and normal colonic mucosa. Methods Immunohistochemistry was used to detect the positive rates of Beclin1 and p62 in 42 cases of colon cancer and normal mucosa. The difference of Beclin1 and p62 expression in different pathological data was compared, and the 5-year survival rate was followed up. The risk factors of death in colon cancer patients were analyzed by Cox regression analysis. Using SPSS 20.0 statistical software for statistical analysis, P <0.05 for the difference was statistically significant. Results The positive rate of Beclin1 expression in normal intestinal mucosa was higher than that in cancerous tissues. The positive rate of Beclin1 expression in T1 ~ T2 phase was higher than that in T3 ~ T4 phase. The difference was statistically significant (P <0.05). The expression of p62 protein in cancer tissue increased, and decreased in normal tissues. The difference between the two groups was statistically significant (P <0.05). Cox regression analysis showed that p62 was a risk factor for death in patients with colon cancer. The combination of p62 and Beclin1 was a good predictor of the prognosis of colon cancer. Conclusions The autophagy activity of colon cancer patients is decreased. The expression of Beclin1 and CEA are correlated with the depth of tumor invasion. The combination of Beclin1 and p62 can predict the prognosis of patients with colon cancer.