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背景:目前国内外关于放射性脑损伤动物模型的研究均处于探索中,尚未形成成熟的模型制作方法。目的:建立急性放射性脑损伤的鼠模型,为进一步研究放射性脑损伤正确有效的预防措施提供实验依据。设计:以实验动物为研究对象,随机对照观察研究。单位:一所大学医院动物实验室。材料:实验于2001-06/2002-08在中山大学附属第二医院实验室完成。SD大鼠60只,体质量(300±30)g,雌雄各半,空白对照组20只,实验组40只。方法:SD大鼠头部接受60Coγ射线照射,7Gy/次,1次/d,连续照射6d,总剂量42Gy。照射结束后每日观察大鼠摄食、饮水量及次数,自主活动情况,有无出现神经系统症状与体征,每周检查并记录大鼠头部照射区毛发与皮肤情况、体质量变化,照射结束后第3,7,14,30天断头取脑,行病理组织学检查。主要观察指标:①一般情况观察。②照射后脑组织病理改变。结果:照射第3天起即出现每日摄食、饮水量减少;照射第1,2天,自主活动较对照组增多,第3天起活动渐减少;无异常神经系统体征;随观察时间延长体质量增长较对照组慢,但差异无显著性意义;所有大鼠均于照射后约2周时出现照射野轻度脱毛;照射后出现脑组织神经元变性坏死。结论:该模型制作方法切实、可靠,较好地模拟放射性脑损伤的过程,可用于预防或减轻放射治疗对脑组织损伤的?
Background: At present, the research on animal models of radiation brain injury both at home and abroad are under exploration. There is no mature model making method yet. Objective: To establish a murine model of acute radiation-induced brain injury and provide experimental evidence for further research on the correct and effective preventive measures for radiation-induced brain injury. Design: The experimental animals as the research object, randomized controlled observation study. Unit: a university hospital animal laboratory. Materials: The experiment was performed at the laboratory of the Second Affiliated Hospital of Sun Yat-sen University from June 2001 to August 2002. 60 SD rats, body weight (300 ± 30) g, male and female, blank control group of 20, experimental group of 40. Methods: The head of SD rats were irradiated with 60Co γ ray, 7Gy / time, once a day for 6 days, the total dose was 42Gy. After inoculation, the daily ingestion, water intake, number of spontaneous activity, presence or absence of nervous system symptoms and signs, weekly examination and record of the rat head irradiation hair and skin conditions, body weight changes, irradiation ended 3,7,14,30 days after decapitation brain, pathological examination. MAIN OUTCOME MEASURES: ① General observation. ② pathological changes of brain tissue after irradiation. Results: Daily feeding started on the third day of exposure and the amount of drinking water decreased. On the first day and the second day of irradiation, the number of autonomous activities increased more than that of the control group and decreased gradually from the third day. No signs of abnormal nervous system were observed. The quality growth was slower than that of the control group, but the difference was not significant. All the rats appeared mild dehairing of the irradiation field at about 2 weeks after irradiation. Degeneration and necrosis of the neurons in the brain tissue occurred after irradiation. Conclusion: The method of making the model is practical, reliable and well simulated the process of radiation brain injury, which can be used to prevent or reduce the damage of brain tissue by radiation therapy.