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１３１例非霍奇金淋巴瘤（ＮＨＬ）按国际工作分类分低、中、高度恶性（ＬＧ、ＭＧ、ＨＧ）３组。对ＮＨＬ和１４例反应性增生淋巴组织（ＲＬＨ）作核分裂计数（ＭＦ），银染核仁组成区（ＡｇＮＯＲｓ）计数，并用ＭＢＣ法检测部分病例的增殖细胞核抗原（ＰＣＮＡ）和Ｋｉ＿６７抗原。ＭＦ、ＡｇＮＯＲｓ、ＰＣＮＡ及Ｋｉ＿６７检测结果有较好相关性（ｒ值０．６１２，Ｐ＜０．０１），均和ＮＨＬ恶性度呈正相关，ＬＧ、ＭＧ、ＨＧ及ＲＬＨ组间有显著性差异（Ｐ＜０．０１，但ＬＧ和ＲＬＨ组Ｐ＞０．０５），表明细胞增殖活性的检测有助于了解ＮＨＬ恶性度，但不能区分ＬＧ和ＲＬＨ。ＭＧ中弥漫小裂细胞型（ＤＳＣ）细胞增殖活性低于同组其它类型（Ｐ＜０．０５），但与ＬＧ无显著性差异（Ｐ＞０．０５），预后相对较好，拟属ＬＧ。ＭＦ、ＡｇＮＯＲｓ和ＰＣＮＡ值越高，生存期越短，表明细胞增殖活性有助于预测ＮＨＬ预后。ＭＦ和ＡｇＮＯＲｓ经济易行；Ｋｉ６７全面反映增殖细胞的数量，仅用于冰冻切片；ＰＣＮＡ可用于石蜡切片，便于回顾性研究。
131 cases of non-Hodgkin’s lymphoma (NHL) were classified into 3 groups according to international work, including low, medium and high grade (LG, MG, HG). Nuclear fission count (MF) and AgNORs were counted in NHL and 14 cases of reactive hyperplasia lymphoid tissue (RLH). PCNA and Ki_67 in some cases were detected by MBC method. MF, AgNORs, PCNA and Ki_67 were significantly correlated (r = 0.612, P <0.01), and were positively correlated with malignancy of NHL. There was significant difference between LG, MG, HG and RLH groups P <0.01, but P <0.05 in LG and RLH groups), indicating that the detection of cell proliferation activity can help to understand the NHL malignancy, but can not differentiate between LG and RLH. The proliferative activity of diffuse small cell type (DSC) cells in MG group was lower than that in other groups (P <0.05), but it was not significantly different from that in LG group (P> 0.05) . The higher MF, AgNORs, and PCNA values, the shorter survival, suggesting that cell proliferative activity is helpful in predicting NHL prognosis. MF and AgNORs are economical; Ki67 fully reflects the number of proliferating cells and is only used for frozen section; PCNA can be used for paraffin section for retrospective study.