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ALVAC是金丝雀痘病毒减毒株的蚀斑纯化分离株,本文作者用基因工程技术构建了该病毒与乙型脑炎病毒(JEV)的重组体,并研究了重组体与一种含prM/M和E蛋白的细胞外亚病毒颗粒(EP)联合免疫的效果.作者将在供体质粒pSPJEVL14VC和pJEV27上产生vP555和vP829的JEV核酸序列通过多点侧链连接插入ALVAC供体质粒中,构建成CP1-20和CP5质粒,分别含有编码prM/M、E、NS1和NS2A的序列,以及编码prM/M和E的序列.这两种质粒被
ALVAC is a plaque-purified isolates of canarypox virus attenuated strains. The authors constructed a recombinant of the virus and Japanese encephalitis virus (JEV) by genetic engineering, and studied the relationship between the recombinant and a prM / M and E. The JEV nucleic acid sequences of vP555 and vP829 produced on donor plasmids pSPJEVL14VC and pJEV27 were inserted into the ALVAC donor plasmid by multipoint side-chain ligation, Constructed into CP1-20 and CP5 plasmids containing sequences encoding prM / M, E, NS1 and NS2A, respectively, and sequences encoding prM / M and E. These two plasmids were