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Objective:To exploretheinfluenceof HBVinvasionintoperipheralbloodmononuclearcells(PBMC)on theimmuneresponseof patientswithchronichepatitisB.Method s:Thecytokinelevelsintheculturesupernatantof PBMCfrom56patientswithchronichepatitisB weredeterminedby ELISA,andPCRwasemployedto amplifythe HBVDNA.Results:Thelevelsof IFN-γinpatientswithhepatitisB waslowerthanthosetof thecontrol,butthe differencewasnotstatisticallysignificant,whilethelevelsof IL-4weresignificantlyhigherthanthoseof thecontrol(P<0.01).Theserumlevelsof HBVDNAwerenegativelycorrelatedwiththatof IFN-γin culturesupernatantsof PBMC.Thirty-fivepatientspositiveof HBVDNA inthePBMCswereidentifiedfrom56patientswithhepatitisB,andtheirIFN-γlevelprovedto be significantlydifferent.Conclusions:Th2cell-mediatedimmuneresponseis predo-minantin chronichepatitisB whichis associatedwiththechronicityof HBVinfection.HBVinvasionintothe PBMCsmayaffectTh1andTh2cell-mediatedimmuneresponseof thepatientswithchronichepatitisB.
Objective: To exploretheinfluenceof HBVinvasionintoperipheralbloodmononuclearcells (PBMC) on theimmuneresponseof patientswithchronichepatitisB.Method s: Thecytokinelevelsintheculturesupernatantof PBMCfrom56patientswithchronichepatitisB weredeterminedby ELISA, andPCRwasemployedto amplifythe HBVDNA.Results: Thelevelsof IFN-γinpatientswithhepatitisB waslowerthanthosetof thecontrol, butthe differencewasnotstatisticallysignificant, whilethelevelsof IL-4weresignificantlyhigherthanthoseof thecontrol (P <0.01) .Theserumlevelsof HBVDNAwerenegativelycorrelatedwiththatof IFN -γin culturesupernatants of PBMC.Thirty-fivepatientspositiveof HBVDNA inthePBMCsWereidentifiedfrom56patientswithhepatitisB, andtheirIFN-γlevelprovedtobe significantlydifferent.Conclusions: Th2cell-mediatedimmuneresponseis predo-minantinchronichepatitisB whichis associatedwiththechronicityof HBVinfection. HBVinvasionintothe PBMCsmayaffectTh1andTh2cell -mediatedimmuneresponseofthepatientswithchronichepatitisB.