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设计合成了基于2,9-双苯并噻唑乙烯基取代的邻菲咯啉荧光分子PMBT。通过吸收光谱、荧光光谱、圆二色谱探讨了PMBT与不同DNA的相互作用,发现PMBT与DNA存在两种不同的相互作用模式。由于PMBT分子中带有2个正电荷,当PMBT与DNA的浓度比值较高(大于4)时,PMBT以DNA为模板按一定方向在DNA上聚集;当PMBT与DNA的浓度比小于2时,PMBT通过嵌插或末端堆积的方式分别与单/双链DNA和G-四链体DNA结合。PMBT与DNA结合导致其荧光猝灭,利用该特性将PMBT与DNA结合构建荧光增强型检测平台,可用于DNA酶活性以及DNA降解的动力学研究。
Design and synthesis of 2,9-bis-benzothiazole vinyl-substituted phenanthroline fluorescent molecule PMBT. The interaction between PMBT and different DNAs was investigated by absorption, fluorescence and circular dichroism. It was found that there are two different interaction modes between PMBT and DNA. Due to the two positive charges in PMBT molecule, when the concentration ratio of PMBT to DNA is higher (greater than 4), PMBT accumulates on DNA with DNA as a template in a certain direction. When the concentration ratio of PMBT to DNA is less than 2, PMBTs bind to single / double-stranded DNA and G-quadruplex DNA, respectively, by insertion or end-stacking. The combination of PMBT and DNA leads to fluorescence quenching. By using this characteristic, PMBT and DNA are combined to form a fluorescence-enhanced detection platform, which can be used for studying the kinetics of DNA enzyme activity and DNA degradation.