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采用二氧化碳(CO2)和强酸(HCl)模拟海水酸化,研究未来海水酸化对马粪海胆(Hemicentrotus pulcherrimus)胚胎早期发育的影响。根据IPCC预测,实验设置了1个自然海水组、3个CO_2酸化胁迫组:OA1-CO_2(自然海水pH降0.3)、OA_2-CO_2(自然海水pH降0.4)和OA_3-CO_2(自然海水pH降0.5));3个HCl酸化胁迫组:OA_1-HCl(自然海水pH降0.3)、OA_2-HCl(自然海水pH降0.4)和OA_3-HCl(自然海水pH降0.5)。结果显示:1两种海水酸化模式对马粪海胆受精率并无显著影响(P>0.05);2与自然海水组相比,两种酸化模式下马粪海胆胚胎上浮率均呈下降趋势,其中OA_1-CO_2组和OA_1-HCl组胚胎上浮率显著降低且差异显著(P<0.05),其余各酸化胁迫组胚胎上浮率则呈极显著降低趋势(P<0.01);3随着酸化程度的加深,两种酸化模式下马粪海胆四腕浮游幼体存活率较自然海水组均呈下降趋势,与自然海水组相比,OA1-HCl组存活率显著降低(P<0.05),其余各酸化胁迫组存活率降低程度极为显著(P<0.01);4与自然海水组相比,CO_2酸化胁迫组马粪海胆四腕浮游幼体的对称性缺失现象随酸化程度的加深而加剧,而HCl酸化胁迫对马粪海胆四腕浮游幼体对称性并无明显影响。结果提示,未来海水酸化对马粪海胆早期发育具有重要影响,与HCl酸化模式相比,由CO_2导致的海水酸化对马粪海胆胚胎早期发育的影响更为复杂。
Carbon dioxide (CO 2) and strong acid (HCl) were used to simulate seawater acidification to study the effect of future sea acidification on the early embryo development of Hemicentrotus pulcherrimus. According to the IPCC prediction, one natural seawater group and three CO_2 acidification stress groups were set up: OA1-CO_2 (natural seawater pH0.3), OA_2-CO_2 (natural seawater pH0.4) and OA3-CO_2 (natural seawater pH drop 0.5), three HCl-acidified stress groups: OA_1-HCl (natural seawater pH drop 0.3), OA_2-HCl (natural seawater pH 0.4) and OA_3-HCl (natural seawater pH 0.5). The results showed that the two modes of seawater acidification had no significant effect on the sea urchin fertilization rate (P> 0.05). 2 Compared with the natural seawater group, The floating rate of embryos in -CO_2 group and OA_1-HCl group was significantly decreased (P <0.05), and the rest of each acidification stress group was significantly decreased (P <0.01); 3 With the deepening of acidification degree, Compared with the natural seawater group, the survival rate of OA1-HCl group was significantly lower (P <0.05), and the survival rate of the other acidification stress groups was lower than that of the natural seawater group (P <0.01). 4 Compared with the natural seawater group, the phenomenon of symmetry deletion of four planktonic horseshoe crab aquatic plants aggravated with the degree of acidification under the condition of CO 2 acidification stress. However, There is no significant effect on the symmetry of four-wrist planktonic larvae. The results suggest that the future acidification of seaweed has an important influence on the early development of horse sea urchin. Compared with the HCl acidification pattern, the effect of seawater acidification by CO 2 on the early embryo development is more complicated.