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目的联合应用MCV、MCH、高效液相色谱(HPLC)方法筛查血红蛋白E(HbE)。方法收集EDTA2K2抗凝血,先行血常规检测平均红细胞体积(MCV)和平均红细胞血红蛋白含量(MCH)初筛,再行HPLC检测HbE,如HPLC结果提示为HbE,进一步行PCR-RBD检测。结果 60例HbE的初筛结果为HBG 122.18±18.37g/L,MCV 76.76±4.19 fl,MCH 25.01±1.72pg;HPLC筛查结果为:HbA2/E洗脱峰含量为26.35±3.16%,滞留时间为3.69±0.03min;基因确诊结果为αα/αα,HbE 52例,合并α地贫8例,HPLC结果与基因诊断结果符合率为100%。结论联合应用MCV、MCH、HPLC法是筛选HbE的有效方法。
Objective To screen hemoglobin E (HbE) by MCV, MCH and high performance liquid chromatography (HPLC). Methods EDTA2K2 anticoagulant blood was collected. The blood samples were collected for the first time in routine blood routine examination and the mean hemoglobin (MCH) were detected by HPLC. HbE was detected by HPLC. HbE was detected by HPLC, and further detected by PCR-RBD. Results The screening results of HbE in 60 patients were 122.18 ± 18.37g / L for HBG, 76.76 ± 4.19 fl for MCV and 25.01 ± 1.72pg for MCH. The results of HPLC screening showed that the peak of HbA2 / E was 26.35 ± 3.16% Was 3.69 ± 0.03min. The results of gene diagnosis were αα / αα, 52 cases of HbE and 8 cases of α-thalassemia. The coincidence rate of HPLC and gene diagnosis was 100%. Conclusion The combined application of MCV, MCH, HPLC method is an effective method for screening HbE.