[Object] The study was to provide a reference for organ development and molecular study. [Method] Fritillaria ussuriensis Maxim. was used as an experiment material, total soluble sugar, reducing sugar and starch contents of tissue culture as indexes, the metabolism of carbohydrates in cultures of Fritllaria ussuriensis Maxim. during different stages of tissue culture were studied. [Result] The total soluble sugar and reducing sugar contents showed decrease and then increase trend in cultures of Fritillaria ussuriensis during different stages, and reached their maximum of 53.41 and 9.23 mg/g at a bulblet growing stage (bulblet-2). Total starch contents showed increase-decrease-increase trend in cultures. At callus stage reached their minimum of 42.32 mg/g, and reached maximum of 84.06 mg/g at a bulblet expanding stage (bulblet-2). [Conclusion] The mechanism of the metabolism of carbohydrates was verified, which provided a theoretical basis for organ development. [Object] The study was to provide a reference for organ development and molecular study. [Method] Fritillaria ussuriensis Maxim. Was used as an experiment material, total soluble sugar, reducing sugar and starch contents of tissue culture as indexes, the metabolism of carbohydrates in cultures of Fritllaria ussuriensis Maxim. during different stages of tissue culture were studied. [Result] The total soluble sugar and reducing sugar contents showed decrease and then increase trend in cultures of Fritillaria ussuriensis during different stages, and reached their maximum of 53.41 and 9.23 At callus stage reached their minimum of 42.32 mg / g, and reached maximum of 84.06 mg / g at a built-in concentration of mg / g at a built-growth stage (bulblet-2) expanding stage (built-2). [Conclusion] The mechanism of the metabolism of carbohydrates was verified, which provides a theoretical basis for organ development.