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目的探索中药红景天有效成分—红景天甙在促进NOS的活性及增加老龄大鼠阴茎组织NO含量中的作用。方法通过对不同年龄大鼠主动脉内皮细胞(RAEC)和大鼠阴茎海绵体平滑肌细胞(RPCSMC)的培养及鉴定,综合运用不同方法测定NO、NOS在不同细胞、不同年龄阶段、不同药物干预浓度和时间作用下的表达。结果以48h为例,2、8、12月龄大鼠阴茎海绵体平滑肌细胞NO含量和NOS活性以及大鼠动脉内皮细胞NO含量和NOS活性间存在显著差异(P<0.01)。在红景天有效药物作用浓度(2.5mg)和有效作用时间(48h、12月龄为72h)下,老龄大鼠(8、12月龄)RPCSMC和RAEC释放NO的量(97.677±5.153)μmol/L,(97.457±3.594)μmol/L及(98.248±5.0851)μmol/L和NOS的量(1.778±0.066)U/mL,(1.738±0.074)U/mL,(1.773±0.044)U/mL和(1.792±0.046)U/mL,较正常年龄(2月龄)大鼠NO和NOS的量还略有升高(P<0.01)。结论(1)阴茎组织中海绵体平滑肌细胞中存在大量NOS,说明阴茎勃起与其自身海绵体产生NO有关;(2)年龄相关性ED与NOS不断减少从而引起NO释放减少密切相关;(3)红景天对AEC及SMC产生NO有积极促进作用,特别是对老龄大鼠细胞作用显著。
Objective To explore the role of salidroside, an active ingredient of Rhodiola sinensis, in promoting the activity of NOS and increasing the NO content in the penis of aged rats. Methods By using different methods to determine the concentration of NO and NOS in different cells, different ages, and different drug interventions, the different concentrations of rat aortic endothelial cells (RAEC) and rat penile cavernosal smooth muscle cells (RPCSMC) were cultured and identified. Expression under the influence of time. Results For 48 hours, there were significant differences in NO content and NOS activity in smooth muscle cells of rat corpus cavernosum, NO content and NOS activity in rat artery endothelial cells at 2, 8 and 12 months of age (P<0.01). The amount of NO released from RPCSMC and RAEC was (97.677±5.153)μmol in aged rats (8 and 12 months of age) at the effective concentration of Rhodiola rosea (2.5 mg) and effective duration (48h, 12 months of 72h). /L, (97.457±3.594) μmol/L and (98.248±5.0851) μmol/L and the amount of NOS (1.778±0.066) U/mL, (1.738±0.074) U/mL, (1.773±0.044) U/mL And (1.792±0.046) U/mL, compared with the normal age (2 months) rats NO and NOS amount also increased slightly (P <0.01). Conclusion (1) There are a lot of NOS in corpus cavernosal smooth muscle cells, which indicates that penile erection is related to the production of NO in their own cavernous body; (2) Age-related ED and NOS are continuously reduced, which leads to the reduction of NO release; (3) Red Sedum has a positive effect on the production of NO by AEC and SMC, especially on the cells of aged rats.