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用佛波酯（佛波醇－１２－豆蔻酸－１３－乙酸酯，ＰＭＡ）作用大鼠肝癌ＣＲＢＨ７９１９细胞，发现ＰＭＡ能促进ＣＲＢＨ７９１９细胞中磷脂酰胆碱（ＰＣ）水解，此效应出现在ＰＭＡ作用细胞１５分钟后，且具有ＰＭＡ浓度依赖性。分析ＰＣ水解产物，发现ＰＣ主要水解生成胆碱而不是磷酸胆碱。进一步测定膜结合磷脂酰胆碱专一性的磷脂酶Ｄ（ＰＣ－ＰＬＤ）活性，结果显示１００ｎｍｏｌ／ＬＰＭＡ作用细胞１０分钟即激活ＰＣ－ＰＬＤ，至３０分钟ＰＣ－ＰＬＤ活力增加至３．２５倍。ＰＭＡ激活ＰＣ－ＰＬＤ在时间上先于ＰＣ水解，提示在ＣＲＢＨ７９１９细胞中ＰＭＡ通过ＰＣ－ＰＬＤ水解ＰＣ产生磷脂酸和胆碱。
Effect of phorbol ester (phorbol 12-myristate-13-acetate, PMA) on rat hepatocellular carcinoma CRBH7919 cells found that PMA can promote the hydrolysis of phosphatidylcholine (PC) in CRBH7919 cells in PMA After 15 minutes, cells were PMA concentration-dependent. Analysis of PC hydrolysates revealed that PC was predominantly hydrolyzed to choline rather than phosphocholine. Further determination of membrane-bound phosphatidylcholine-specific phospholipase D (PC-PLD) activity showed that 100 nmol / LPMA activated cells activated PC-PLD for 10 min and increased PC-PLD viability by 3.25 to 30 min . PMA-activated PC-PLD precedes PC hydrolysis in time, suggesting that PMA hydrolyzes PC by PC-PLD in CRBH7919 cells to produce phosphatidic acid and choline.