论文部分内容阅读
应用琼酯糖－纤维蛋白－平板法和抗人尿激酶多克隆抗体的免疫阻断法，分别测定了２０名正常生育力男子和２２例精子活力低下（弱精症）的男性不育症患者精浆中及精子膜尿激酶型纤溶酶原激活因子（ｕＰＡ）的酶活性。结果：弱精症组精浆ｕＰＡ活性为２１３４±１５８１ＩＵ／Ｌ，正常生育组精浆ｕＰＡ活性为３３６５±１８５９．５ＩＵ／Ｌ，两组间差异有显著性意义（Ｐ＜０．０５）。弱精症组精子膜ｕＰＡ活性为５．１３±３．８２ｍＵ／１０６ｃｅｌｓ，正常生育组精子膜ｕＰＡ活性为１０．１７±６．１８ｍＵ／１０６ｃｅｌｓ，两组间差异有极显著性意义（Ｐ＜０．００５）。精子膜ｕＰＡ活性值与精子活率、活力亦呈线性相关。提示精子膜ｕＰＡ酶活性与精子活力及生育力可能有一定关系。
20 normal fertility men and 22 male infertility patients with low sperm motility (asthenospermia) were measured by agarose-fibrin-plate method and anti-human urokinase polyclonal antibody blocking method Seminal plasma and sperm membrane urokinase type plasminogen activator (uPA) activity. Results: The uPA activity of seminal plasma in asthenospermia group was 2134 ± 1581 IU / L. The activity of uPA in normal fertility group was 3365 ± 1859.5 IU / L, with significant difference between the two groups (P <0.05). The uPA activity of sperm membrane in the asthenospermia group was 5.13 ± 3.82mU / 106cels, and the uPA activity in normal fertility group was 10.17 ± 6.18mU / 106cels. There was significant difference between the two groups (P <0. 05) .005). Sperm membrane uPA activity and sperm motility, vitality also showed a linear correlation. Tip sperm membrane uPA enzyme activity and sperm motility and fertility may have a certain relationship.