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目的 :观察吸入不同浓度富氧气体对 110 0 0m低压条件下大耳白兔肾EPO基因表达的影响 ,从分子生物学水平为机载分子筛制氧系统的应用提供理论依据。方法 :2 4只大耳白兔随机分为 4组 (n =6 ) :空气对照组 ,6 3%富氧气体组 ,83%富氧气体组 ,纯氧组。将各组动物置于低压舱内 ,供给不同浓度的富氧气体 ,低压舱上升到 110 0 0m ,停留 30min ,然后将动物即刻处死 ,提取肾组织总RNA ,用RT -PCR方法检测肾组织EPOmRNA表达情况。结果 :大耳白兔暴露于 110 0 0m后 ,空气对照组EPOmRNA表达的阳性比率为 6 / 6 ,6 3%富氧气体组为 4 / 6 ,83%富氧气体组为 3/ 6 ,纯氧组为 1/ 6。随着吸入气氧浓度的增加 ,EPOmRNA表达的阳性率降低。吸氧浓度与EPO基因表达的阳性率呈明显负相关 (r =- 0 .976 0 4 ,P <0 .0 5 )。结论 :吸入较高浓度富氧气体对 110 0 0m低压下大耳白兔EPO基因的表达有明显的抑制作用
OBJECTIVE: To observe the effects of different concentrations of oxygen-enriched gas inhalation on the expression of renal EPO gene in rabbits at 110 0 m low pressure, and to provide a theoretical basis for the application of oxygen molecular oxygen system to airborne molecular sieves. Methods: Twenty four rabbits were randomly divided into four groups (n = 6): air control group, 63% oxygen-enriched gas group, 83% oxygen-enriched gas group and pure oxygen group. Each group of animals was placed in the low-pressure chamber, supplied with different concentrations of oxygen-enriched gas, low pressure tank up to 110 0m, stay 30min, and then the animals were sacrificed immediately, total RNA was extracted from renal tissue, RT-PCR detection of renal tissue EPOmRNA Express the situation. Results: The positive rate of EPO mRNA expression in air control group was 6/6 after exposed to 110 000 m, the ratio of EPO mRNA expression in air-enriched group was 4/6, that of 83% oxygen-enriched gas group was 3/6 Oxygen group was 1/6. With the increase of inhaled oxygen concentration, the positive rate of EPO mRNA expression decreased. Oxygen concentration was negatively correlated with the positive rate of EPO gene expression (r = -0.976 0 4, P <0.05). Conclusion: Inhalation of oxygen-enriched gas at higher concentration significantly inhibited the expression of EPO gene in large white rabbits at 110 0 m