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以ｍｅｓｏ－四（４－三甲铵基苯基）－卟啉（Ｈ２ＴＡＰＰ）为柱前衍生试剂，建立了一种简便、快速分离和测定Ｃｕ（Ⅱ）和Ｚｎ（Ⅱ）的高效液相色谱法。３分钟内，以甲醇／水＝４０／６０（Ｖ／Ｖ）为洗脱液，在Ｓｈｉｍ—ｐａｃｋＷＣＸ—１（５μｍ，５０×４．０ｍｍｉ．ｄ．）柱上成功地分离并测定Ｃｕ（Ⅱ）和Ｚｎ（Ⅱ），检测极限可达５．０×１０－１０ｍｏｌ／Ｌ。以含４０％甲醇的乙二胺缓冲液为洗脱剂，Ｈ２ＴＡＰＰ及其金属配合物在ＳｐｅｒｉｓｏｒｂＳＣＸ柱（１０μｍ，２５０×４．０ｍｍｉ．ｄ．）和Ｓｈｉｍ—ＰａｃｋＷＣＸ－１柱上的离子交换色谱行为也被考察。该方法具有灵敏度高，操作简便的特点，可用于样品中痕量钢、锌的分析。
A simple and rapid method for the separation and determination of Cu (Ⅱ) and Zn (Ⅱ) was developed using meso-tetrakis (4-trimethylammoniumphenyl) -porphyrin (H2TAPP) as a pre-column derivatization reagent . The Cu (II) complex was successfully separated and quantified on a Shim-pack WCX-1 (5 [mu] m, 50 x 4.0 mm ID) column with a methanol / water = 40/60 (V / ) And Zn (Ⅱ), the detection limit of up to 5.0 × 10-10mol / L. Ion exchange chromatography of H2TAPP and its metal complexes on a SperisorbSCX column (10μm, 250 × 4.0mmi.d.) And Shim-PackWCX-1 column using 40% methanol in ethylenediamine buffer as eluent Also investigated. The method has the characteristics of high sensitivity and easy operation, and can be used for the analysis of trace steel and zinc in the sample.