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目的基于“肝藏”理论,通过对肝郁血虚ED大鼠的观察,探讨“疏肝养血法”调控阴茎海绵体平滑肌的物质基础与效应机制。方法将30只SD大鼠随机分3组,即空白组、模型组、治疗组,每组各10只。治疗组予以四物汤加逍遥散合方治疗,空白组、模型组给予等体积生理盐水,连续14d。观察大鼠性活动情况、阴茎海绵体平滑肌形态及钙离子通道蛋白CaV1.3、RyR1的表达。结果(1)与空白组比较,模型组大鼠性活动能力明显减弱,即骑跨潜伏期[(158.16±27.42)s]、插入潜伏期[(525.23±105.56)s]延长,骑跨次数[35.31±6.17]与插入次数[8.84±1.48]明显减少(P<0.05);与模型组比较,治疗组骑跨潜伏期[(96.07±18.26)s]、插入潜伏期[(310.27±104.24)s]缩短,骑跨次数[49.6±18.65]与插入次数[15.21±4.63]显著改善(P<0.05)。(2)各组阴茎海绵体平滑肌病理切片HE染色后,形态学改变不明显(P>0.05)。(3)与空白组比较,模型组大鼠阴茎海绵体平滑肌CaV1.3[22059.51±10945.64]及RyR1蛋白[25053.70±2941.02]表达显著降低(P<0.05);与模型组比较,治疗组CaV1.3[42386.31±9515.26]及RyR1蛋白[38038.25±4980.82]表达显著改善(P<0.05)。结论疏肝养血法可显著改善肝郁血虚ED大鼠阴茎组织Ca2+通道蛋白CaV1.3及RyR1的表达,可能是“肝藏”的理论物质基础及效应机制的重要环节。
Objective Based on the theory of “liver reservoir”, through the observation of ED rats with liver-qi deficiency, we explore the material basis and mechanism of “Shugan Yangxue method” regulating the smooth muscle of the penis. Methods Thirty SD rats were randomly divided into three groups: blank group, model group and treatment group, with 10 rats in each group. The treatment group was treated with Siwutang plus Xiaoyao San, while the blank group and model group were given equal volume of normal saline for 14 days. The rats were observed for sexual activity, the morphology of the corpus cavernosum and the expression of Ca (superscript 3 +) and RyR1. Results (1) Compared with the blank group, the ability of sexual activity in the model group was significantly weakened, that is, the latency of riding-in was [158.16 ± 27.42] s, the latency of insertion was (525.23 ± 105.56) s] 6.17] and insertion times [8.84 ± 1.48] (P <0.05). Compared with the model group, the latencies of the treatment group were shorter (96.07 ± 18.26 s) and insertion latency The number of intercrosses [49.6 ± 18.65] and insertion number [15.21 ± 4.63] were significantly improved (P <0.05). (2) The pathological changes of smooth muscle of corpus cavernosum in each group were not obvious after HE staining (P> 0.05). (3) Compared with the blank group, the expression of CaV1.3 [22059.51 ± 10945.64] and RyR1 protein [25053.70 ± 2941.02] in the model group was significantly decreased (P <0.05). Compared with the model group, the CaV1 in the treatment group was significantly decreased. 3 [42386.31 ± 9515.26] and RyR1 protein [38038.25 ± 4980.82] were significantly improved (P <0.05). Conclusion Shugan Yangxue Therapy can significantly improve the expression of Ca2 + channel proteins Ca1 + and RyR1 in penile tissue of ED rats with liver-qi deficiency and may be an important part of the theoretical material basis and mechanism of effect of “liver-hidden”.