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本文采用 ATP 酶染色法,连续、动态观察紫外线照射后豚鼠表皮内郎格罕细胞(LC)的密度及结构变化。结果表明:波长200～400nm,剂量422.4～1689.6mJ/cm~3紫外线照射豚鼠皮肤后,局部 LC 密度明昴降低(P<0.01),细胞数目较照射前减少28～51%。细胞突起减少、变短、部分细胞变圆,体积增大,胞浆呈空泡状。皮肤冰冻切片(H—E)见表皮增厚,表层细胞角化明显。停止照射后8d,郎格罕细胞密度及结构基本恢复正常。文中对紫外线照射使表皮内郎格罕细胞数目减少的原因及其临床意义进行了讨论。
In this study, ATPase staining was used to observe the density and structural changes of Langerhans cells (LC) in the epidermis of guinea pigs after UV irradiation. The results showed that after irradiation of guinea pig skin with the wavelength of 200-400nm and dose of 422.4-1689.6mJ/cm~3, the local density of LC was decreased (P<0.01), and the number of cells decreased by 28~51% compared with that before irradiation. The cell protrusions decreased, became shorter, some cells became round, and the volume increased, and the cytoplasm was vacuolated. Skin epidermal thickness (H-E) showed epidermal thickening, apparent keratosis of the surface cells. After 8 days of radiation, Langerhans cell density and structure returned to normal. In this paper, the causes of the decrease of Langerhans cell number in the epidermis by ultraviolet irradiation and its clinical significance are discussed.