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恶病质是肿瘤生长致宿主消耗为主的临床综合征.宿主的组织分解为肿瘤合成蛋白质提供底物和能量.有实验表明静脉和肠道补充蛋白质能选择性刺激肿瘤生长,而对生存无益.因此,对肿瘤患者的蛋白质支持颇有争论.生长激素(GH)已被证实在各种分解状态能刺激肌肉合成蛋白质,改善氮平衡.GH是否刺激实体瘤增长还未见报道.MAC-33肿瘤是一种特发肺转移的乳腺腺癌,接种后的第15～25天出现肺转移,能致宿主恶病质,但不引起厌食.本实验对40只Lewis雌鼠,于右胁腹皮下接种10~6MAC-33细胞,待注射后第10天,一出现肿瘤即分为四组.Ⅰ组喂标准鼠食;Ⅱ组喂标准鼠食加GH(每晨注射GH1IU/kg/天);Ⅲ组喂去蛋白食物(仅含0.16%蛋白质);Ⅳ组喂去蛋白食物加GH.第35天处死所有鼠.测定瘤体积,体重,处死时心脏抽血测定氨基酸序列.对肿瘤,肝脏、腘绳肌均进行DNA、RNA和蛋白质量测定.鼠体重减去瘤重等于尸体的重量.结果:去蛋白质食物组(Ⅲ组)与标准食物组
Cachexia is a clinical syndrome characterized by tumor growth and host depletion. Host tissue breakdown provides substrates and energy for tumor synthesis proteins. Experiments have shown that venous and enteral protein supplements can selectively stimulate tumor growth without benefiting survival. There is considerable controversy over protein support in cancer patients. Growth hormone (GH) has been shown to stimulate muscle synthesis of protein in various decomposing states and improve nitrogen balance. GH has not been reported to stimulate solid tumor growth. MAC-33 tumors are An adenocarcinoma of the breast with metastatic lung metastases. Lung metastasis occurs 15 to 25 days after inoculation and can cause host cachexia without causing anorexia. In this experiment, 40 Lewis females were inoculated subcutaneously in the right flank 10~. 6MAC-33 cells, the first 10 days after injection, a tumor was divided into four groups. Group I was fed standard rat food; Group II was fed standard rat food plus GH (every morning injection of GH1IU/kg/day); Group III fed Deproteinized food (containing only 0.16% protein); Group IV fed deproteinized food plus GH. All rats were sacrificed on the 35th day. The volume of the tumor was measured, body weight, cardiac blood was drawn at the time of sacrifice to determine the amino acid sequence. On the tumor, liver, and hamstring All DNA, RNA, and protein quality measurements were taken. Tumor weight equal to the weight of the bodies results: protein foods to group (Ⅲ group) and the standard feed group